Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene

Yi Chung Chen, Chia Hsiung Cheng, Gen Der Chen, Chin Chun Hung, Chung Hsiang Yang, Sheng Ping L Hwang, Koichi Kawakami, Bo Kai Wu, Chang Jen Huang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Human synuclein family consists of α-, β-, and γ-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode β-, γ1-, and γ2-synucleins, respectively. The zSyn-γ, zSyn-γ1, and zSyn-γ2 proteins display 69%, 47%, and 50% identity to human β-synuclein and γ-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5′-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain.

Original languageEnglish
Pages (from-to)746-754
Number of pages9
JournalDevelopmental Dynamics
Volume238
Issue number3
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Fingerprint

Synucleins
Zebrafish
Green Fluorescent Proteins
Reporter Genes
Brain
Habenula
Genes
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Genetic Promoter Regions
Ganglia
Testis
Ovary
Central Nervous System
Kidney
Proteins

Keywords

  • Habenular nuclei
  • Interpeduncular nucleus
  • Promoter
  • Synuclein
  • Tol2-mediated transgenesis
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. / Chen, Yi Chung; Cheng, Chia Hsiung; Chen, Gen Der; Hung, Chin Chun; Yang, Chung Hsiang; Hwang, Sheng Ping L; Kawakami, Koichi; Wu, Bo Kai; Huang, Chang Jen.

In: Developmental Dynamics, Vol. 238, No. 3, 03.2009, p. 746-754.

Research output: Contribution to journalArticle

Chen, Yi Chung ; Cheng, Chia Hsiung ; Chen, Gen Der ; Hung, Chin Chun ; Yang, Chung Hsiang ; Hwang, Sheng Ping L ; Kawakami, Koichi ; Wu, Bo Kai ; Huang, Chang Jen. / Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. In: Developmental Dynamics. 2009 ; Vol. 238, No. 3. pp. 746-754.
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