Rearrangements of immunoglobulin gene and oncogenes in ocular adnexal pseudolymphoma

Po Min Chen, Jin Hwang Liu, Siu Huie Lin, Wen-Ming Hsu, Shu Ching Kao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The organization of immunoglobulin heavy chain (IgH), light chain (kappa and lambda) and T cell receptor (TCR) beta chain gene loci in 10 patients with ocular adnexal pseudolymphoma was investigated. Eight of them showed IgH gene rearrangement in at least one of the 3 restriction enzymes-digested DNAs extracted from ocular adnexal neoplasms. In contrast, none of them exhibited clonal TCR beta chain gene rearrangement. The configuration of bcl-1, bcl-2 and c-myc oncogenes was also studied by Southern blot technique. Two patients had a rearranged joining region, IgH-containing fragment that comigrated with the rearranged bcl-1 fragment. C-myc gene rearrangement was found in only one patient who also had bcl-1 gene rearrangement. In ocular adnexal pseudolymphoma, none demonstrated bcl-2 gene rearrangement; however, in bone marrow cells, one patient with systemic lymphadenopathy exhibited both IgH and bcl-2 gene rearrangements. Three genotypic subsets of these ocular adnexal pseudolymphoma can be identified by the configuration of IgH gene and related oncogenes: with germline configuration of IgH gene and bcl-1, bcl-2 and c-myc oncogenes; with rearrangement of IgH gene but germline configuration of these oncogenes; and with recombination between rearranged IgH and bcl-1 genes. These results suggest in ocular adnexal pseudolymphoma a spectrum of clonal change evolving from polyclonal to monoclonal B-population, and further to monoclonal B-population with rearranged bcl-1, c-myc and/or bcl-2 oncogenes.

Original languageEnglish
Pages (from-to)547-555
Number of pages9
JournalCurrent Eye Research
Volume10
Issue number6
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

Fingerprint

Pseudolymphoma
Immunoglobulin Genes
Gene Rearrangement
Immunoglobulin Heavy Chain Genes
Immunoglobulin Heavy Chains
Oncogenes
myc Genes
bcl-1 Genes
T-Cell Receptor beta Genes
bcl-2 Genes
beta-Chain T-Cell Antigen Receptor Gene Rearrangement
Eye Neoplasms
DNA Restriction Enzymes
Southern Blotting
Bone Marrow Cells
Genetic Recombination
Population
Light

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Rearrangements of immunoglobulin gene and oncogenes in ocular adnexal pseudolymphoma. / Chen, Po Min; Liu, Jin Hwang; Lin, Siu Huie; Hsu, Wen-Ming; Kao, Shu Ching.

In: Current Eye Research, Vol. 10, No. 6, 01.01.1991, p. 547-555.

Research output: Contribution to journalArticle

Chen, Po Min ; Liu, Jin Hwang ; Lin, Siu Huie ; Hsu, Wen-Ming ; Kao, Shu Ching. / Rearrangements of immunoglobulin gene and oncogenes in ocular adnexal pseudolymphoma. In: Current Eye Research. 1991 ; Vol. 10, No. 6. pp. 547-555.
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