Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates

Mao Yuan Chen; Chien Ching Hung; Yvonne G. Lin; Ruey Yi Lin; Shing Jer Twu; Ying Chin Ko; Hong Nerng Ho; Chang Ping Lin; Shiu Feng Huang; Mary Ellen Kerrigan; Jacqueline Whang-Peng; Che Yen Chuang; Kenneth S.S. Chang; Juei Low Sung; Hong Jen Chang; Yaw Tang Shih; Po Ya Chang

doi:10.1016/S1201-9712(96)90058-2

Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates

Mao Yuan Chen, Chien Ching Hung, Yvonne G. Lin, Ruey Yi Lin, Shing Jer Twu, Ying Chin Ko, Hong Nerng Ho, Chang Ping Lin, Shiu Feng Huang, Mary Ellen Kerrigan, Jacqueline Whang-Peng, Che Yen Chuang, Kenneth S.S. Chang, Juei Low Sung, Hong Jen Chang, Yaw Tang Shih, Po Ya Chang

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: Serial Western Blot (WB) testing was utilized for the early diagnosis of neonatal HIV infection. Methods: Serum samples included five anti-HIV positive mother/child pairs and 57 newborns who received hepatitis B immunoglobulin and hepatitis B vaccine. Enzyme-linked immunosorbent assay (ELISA) and serial WB were supplemented by polymerase chain reaction (PCR) and gag sequencing studies. Results: Serial WB patterns between the five mothers and their newborns were initially identical. Reaction lines, however, declined progressively and proportionately in the three infants not HIV infected. Two newborns were diagnosed early to be HIV-positive by evaluating the intensifying and increasing WB reaction lines. For the group of newborns who underwent the hepatitis B control program, all WB reaction lines declined progressively with time and none of these infants were truly HIV infected. Conclusion: Senal WB is simple and reliable and may be used for the early diagnosis of HIV infection in infants.

Original language	English
Pages (from-to)	83-86
Number of pages	4
Journal	International Journal of Infectious Diseases
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/S1201-9712(96)90058-2
Publication status	Published - 1996
Externally published	Yes

Keywords

Early diagnosis of HIV infection
HIV/AIDS
Hepatitis B immunoglobulin (HBIG)
Hepatitis B virus (HBV)
Neonatal HIV infection
Western Blot

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1201-9712(96)90058-2

Cite this

Chen, M. Y., Hung, C. C., Lin, Y. G., Lin, R. Y., Twu, S. J., Ko, Y. C., Ho, H. N., Lin, C. P., Huang, S. F., Kerrigan, M. E., Whang-Peng, J., Chuang, C. Y., Chang, K. S. S., Sung, J. L., Chang, H. J., Shih, Y. T., & Chang, P. Y. (1996). Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates. International Journal of Infectious Diseases, 1(2), 83-86. https://doi.org/10.1016/S1201-9712(96)90058-2

Chen, MY, Hung, CC, Lin, YG, Lin, RY, Twu, SJ, Ko, YC, Ho, HN, Lin, CP, Huang, SF, Kerrigan, ME, Whang-Peng, J, Chuang, CY, Chang, KSS, Sung, JL, Chang, HJ, Shih, YT & Chang, PY 1996, 'Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates', International Journal of Infectious Diseases, vol. 1, no. 2, pp. 83-86. https://doi.org/10.1016/S1201-9712(96)90058-2

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title = "Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates",

abstract = "Objective: Serial Western Blot (WB) testing was utilized for the early diagnosis of neonatal HIV infection. Methods: Serum samples included five anti-HIV positive mother/child pairs and 57 newborns who received hepatitis B immunoglobulin and hepatitis B vaccine. Enzyme-linked immunosorbent assay (ELISA) and serial WB were supplemented by polymerase chain reaction (PCR) and gag sequencing studies. Results: Serial WB patterns between the five mothers and their newborns were initially identical. Reaction lines, however, declined progressively and proportionately in the three infants not HIV infected. Two newborns were diagnosed early to be HIV-positive by evaluating the intensifying and increasing WB reaction lines. For the group of newborns who underwent the hepatitis B control program, all WB reaction lines declined progressively with time and none of these infants were truly HIV infected. Conclusion: Senal WB is simple and reliable and may be used for the early diagnosis of HIV infection in infants.",

keywords = "Early diagnosis of HIV infection, HIV/AIDS, Hepatitis B immunoglobulin (HBIG), Hepatitis B virus (HBV), Neonatal HIV infection, Western Blot",

author = "Chen, {Mao Yuan} and Hung, {Chien Ching} and Lin, {Yvonne G.} and Lin, {Ruey Yi} and Twu, {Shing Jer} and Ko, {Ying Chin} and Ho, {Hong Nerng} and Lin, {Chang Ping} and Huang, {Shiu Feng} and Kerrigan, {Mary Ellen} and Jacqueline Whang-Peng and Chuang, {Che Yen} and Chang, {Kenneth S.S.} and Sung, {Juei Low} and Chang, {Hong Jen} and Shih, {Yaw Tang} and Chang, {Po Ya}",

year = "1996",

doi = "10.1016/S1201-9712(96)90058-2",

language = "English",

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pages = "83--86",

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T1 - Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates

AU - Chen, Mao Yuan

AU - Hung, Chien Ching

AU - Lin, Yvonne G.

AU - Lin, Ruey Yi

AU - Twu, Shing Jer

AU - Ko, Ying Chin

AU - Ho, Hong Nerng

AU - Lin, Chang Ping

AU - Huang, Shiu Feng

AU - Kerrigan, Mary Ellen

AU - Whang-Peng, Jacqueline

AU - Chuang, Che Yen

AU - Chang, Kenneth S.S.

AU - Sung, Juei Low

AU - Chang, Hong Jen

AU - Shih, Yaw Tang

AU - Chang, Po Ya

PY - 1996

Y1 - 1996

N2 - Objective: Serial Western Blot (WB) testing was utilized for the early diagnosis of neonatal HIV infection. Methods: Serum samples included five anti-HIV positive mother/child pairs and 57 newborns who received hepatitis B immunoglobulin and hepatitis B vaccine. Enzyme-linked immunosorbent assay (ELISA) and serial WB were supplemented by polymerase chain reaction (PCR) and gag sequencing studies. Results: Serial WB patterns between the five mothers and their newborns were initially identical. Reaction lines, however, declined progressively and proportionately in the three infants not HIV infected. Two newborns were diagnosed early to be HIV-positive by evaluating the intensifying and increasing WB reaction lines. For the group of newborns who underwent the hepatitis B control program, all WB reaction lines declined progressively with time and none of these infants were truly HIV infected. Conclusion: Senal WB is simple and reliable and may be used for the early diagnosis of HIV infection in infants.

AB - Objective: Serial Western Blot (WB) testing was utilized for the early diagnosis of neonatal HIV infection. Methods: Serum samples included five anti-HIV positive mother/child pairs and 57 newborns who received hepatitis B immunoglobulin and hepatitis B vaccine. Enzyme-linked immunosorbent assay (ELISA) and serial WB were supplemented by polymerase chain reaction (PCR) and gag sequencing studies. Results: Serial WB patterns between the five mothers and their newborns were initially identical. Reaction lines, however, declined progressively and proportionately in the three infants not HIV infected. Two newborns were diagnosed early to be HIV-positive by evaluating the intensifying and increasing WB reaction lines. For the group of newborns who underwent the hepatitis B control program, all WB reaction lines declined progressively with time and none of these infants were truly HIV infected. Conclusion: Senal WB is simple and reliable and may be used for the early diagnosis of HIV infection in infants.

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KW - HIV/AIDS

KW - Hepatitis B immunoglobulin (HBIG)

KW - Hepatitis B virus (HBV)

KW - Neonatal HIV infection

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Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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