Reappraisal of the early diagnostic value of serial analyses of Western Blot patterns for HIV infection in Neonates

Mao Yuan Chen, Chien Ching Hung, Yvonne G. Lin, Ruey Yi Lin, Shing Jer Twu, Ying Chin Ko, Hong Nerng Ho, Chang Ping Lin, Shiu Feng Huang, Mary Ellen Kerrigan, Jacqueline Whang-Peng, Che Yen Chuang, Kenneth S.S. Chang, Juei Low Sung, Hong Jen Chang, Yaw Tang Shih, Po Ya Chang

Research output: Contribution to journalArticlepeer-review


Objective: Serial Western Blot (WB) testing was utilized for the early diagnosis of neonatal HIV infection. Methods: Serum samples included five anti-HIV positive mother/child pairs and 57 newborns who received hepatitis B immunoglobulin and hepatitis B vaccine. Enzyme-linked immunosorbent assay (ELISA) and serial WB were supplemented by polymerase chain reaction (PCR) and gag sequencing studies. Results: Serial WB patterns between the five mothers and their newborns were initially identical. Reaction lines, however, declined progressively and proportionately in the three infants not HIV infected. Two newborns were diagnosed early to be HIV-positive by evaluating the intensifying and increasing WB reaction lines. For the group of newborns who underwent the hepatitis B control program, all WB reaction lines declined progressively with time and none of these infants were truly HIV infected. Conclusion: Senal WB is simple and reliable and may be used for the early diagnosis of HIV infection in infants.

Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalInternational Journal of Infectious Diseases
Issue number2
Publication statusPublished - 1996
Externally publishedYes


  • Early diagnosis of HIV infection
  • Hepatitis B immunoglobulin (HBIG)
  • Hepatitis B virus (HBV)
  • Neonatal HIV infection
  • Western Blot

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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