Abstract
Devices based on semiconducting nanowires (NWs) are functioning as highly sensitive and selective sensors for the label-free detection of biological and chemical species. This paper demonstrates a novel back-gated silicon NW field effect transistor (NWFET) for gene detection. The fabricated NWFET was employed as the biomolecule sensor for the early, real-time, and label-free screening of hepatitis B virus (HBV) X gene. The DNA fragment in HBV demonstrates the linearity from 10 fM to 1 pM, of which the detection limit is estimated to be about 3.2 fM. The obtained results also show that the NW-based sensor can distinguish the difference between the complementary and 1-base mismatch DNA. The back-gated NW FET exhibits a label-free, highly sensitive, and selective biosensor for gene detection, which also provides a possibility of multiple chemical and biological species detection with sensor array in an integrated chip.
| Original language | English |
|---|---|
| Pages (from-to) | 273-277 |
| Number of pages | 5 |
| Journal | Journal of Polymer Engineering |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1 2014 |
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Keywords
- field-effect transistor
- hepatitis B virus
- label-free
- nanowire
- X gene
ASJC Scopus subject areas
- Materials Chemistry
- Polymers and Plastics
- Chemical Engineering(all)
Cite this
Real-time sensing of hepatitis B virus X gene using an ultrasensitive nanowire field effect transistor. / Wu, Chia Yu; Cheng, Han Yi; Ou, Keng Liang; Wu, Chi Chang.
In: Journal of Polymer Engineering, Vol. 34, No. 3, 01.05.2014, p. 273-277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real-time sensing of hepatitis B virus X gene using an ultrasensitive nanowire field effect transistor
AU - Wu, Chia Yu
AU - Cheng, Han Yi
AU - Ou, Keng Liang
AU - Wu, Chi Chang
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Devices based on semiconducting nanowires (NWs) are functioning as highly sensitive and selective sensors for the label-free detection of biological and chemical species. This paper demonstrates a novel back-gated silicon NW field effect transistor (NWFET) for gene detection. The fabricated NWFET was employed as the biomolecule sensor for the early, real-time, and label-free screening of hepatitis B virus (HBV) X gene. The DNA fragment in HBV demonstrates the linearity from 10 fM to 1 pM, of which the detection limit is estimated to be about 3.2 fM. The obtained results also show that the NW-based sensor can distinguish the difference between the complementary and 1-base mismatch DNA. The back-gated NW FET exhibits a label-free, highly sensitive, and selective biosensor for gene detection, which also provides a possibility of multiple chemical and biological species detection with sensor array in an integrated chip.
AB - Devices based on semiconducting nanowires (NWs) are functioning as highly sensitive and selective sensors for the label-free detection of biological and chemical species. This paper demonstrates a novel back-gated silicon NW field effect transistor (NWFET) for gene detection. The fabricated NWFET was employed as the biomolecule sensor for the early, real-time, and label-free screening of hepatitis B virus (HBV) X gene. The DNA fragment in HBV demonstrates the linearity from 10 fM to 1 pM, of which the detection limit is estimated to be about 3.2 fM. The obtained results also show that the NW-based sensor can distinguish the difference between the complementary and 1-base mismatch DNA. The back-gated NW FET exhibits a label-free, highly sensitive, and selective biosensor for gene detection, which also provides a possibility of multiple chemical and biological species detection with sensor array in an integrated chip.
KW - field-effect transistor
KW - hepatitis B virus
KW - label-free
KW - nanowire
KW - X gene
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U2 - 10.1515/polyeng-2013-0216
DO - 10.1515/polyeng-2013-0216
M3 - Article
VL - 34
SP - 273
EP - 277
JO - Journal of Polymer Engineering
JF - Journal of Polymer Engineering
SN - 0334-6447
IS - 3
ER -