Reactive oxygen scavenger effect of pyrimidines, benzotriazoles and related compounds

Chun Yu Lin, An Chieh Ho, Hsüdch Ching Chiang, Jui Sheng Sun, Shiow Yunn Sheu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Free radicals and reactive oxygen metabolites have been implicated as important pathologic mediators in many clinical disorders and diseases. An efficient method of detecting the free radical scavenger effect is through xanthine oxidase (XO) inhibition. The inhibition efficiency on XO has been detected as the rate of uric acid production, which has max 295 nm. Sulfasalazine showed potent inhibiting activity on XO (IC50 = 25.11μM; Ki = 50.88μM) and induced a mixed-type (noncompetitive-uncompetitive) inhibition of the substrate xanthine. 2-mercapto-4(3H)-quinazolinone (16) and 2-mercaptopyrimidine (4) displayed inhibiting activity on XO with IC50 = 98.71 and 136.14μM, while apparent inhibition constants (Ki) were 158.38 and 62.46μM, respectively. However benzotriazoles showed weak inhibitory effect. The spin-trapping method with 5,5-dimethyl-l-pyrroline N-oxide (DMPO) by electron spin resonance (ESR) detected the presence of O2-. and OH.. It showed that the percentage inhibition for formation of DMPO-OOH for 2-mercapto-pyrimidine and sulfasalazine were 64.78 and 35.09, but for hydroxylation were 49.51, 38.55, 37.29 for 2-mercapto-4(3H)-quinazolinone, sulfasalazine and 2-mercaptopyrimidine at 500μM, respectively.

Original languageEnglish
Pages (from-to)937-942
Number of pages6
JournalAnticancer Research
Volume22
Issue number2 A
Publication statusPublished - 2002

Fingerprint

Pyrimidines
Xanthine Oxidase
Sulfasalazine
Oxygen
Inhibitory Concentration 50
Spin Trapping
Free Radical Scavengers
Xanthine
Electron Spin Resonance Spectroscopy
Hydroxylation
Uric Acid
Oxides
Free Radicals
benzotriazole
pyrimidine-2-thiol
2-mercapto-4(3H)-quinazolinone

Keywords

  • Benzotriazoles
  • Electron spin resonance
  • Hydroxyl radical
  • Pyrimidines
  • Sulfasalazine
  • Superoxide anion
  • Xanthine oxidase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Reactive oxygen scavenger effect of pyrimidines, benzotriazoles and related compounds. / Lin, Chun Yu; Ho, An Chieh; Chiang, Hsüdch Ching; Sun, Jui Sheng; Sheu, Shiow Yunn.

In: Anticancer Research, Vol. 22, No. 2 A, 2002, p. 937-942.

Research output: Contribution to journalArticle

Lin, CY, Ho, AC, Chiang, HC, Sun, JS & Sheu, SY 2002, 'Reactive oxygen scavenger effect of pyrimidines, benzotriazoles and related compounds', Anticancer Research, vol. 22, no. 2 A, pp. 937-942.
Lin, Chun Yu ; Ho, An Chieh ; Chiang, Hsüdch Ching ; Sun, Jui Sheng ; Sheu, Shiow Yunn. / Reactive oxygen scavenger effect of pyrimidines, benzotriazoles and related compounds. In: Anticancer Research. 2002 ; Vol. 22, No. 2 A. pp. 937-942.
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