Reactive changes of interstitial glia and pinealocytes in the rat pineal gland challenged with cell wall components from gram-positive and -negative bacteria

Ya Fen Jiang-Shieh, Ching Hsiang Wu, Hsiung Fei Chien, I. Hua Wei, Min Lin Chang, Jeng Yung Shieh, Chen Yuan Wen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS), the major proinflammatory component of gram-negative bacteria, is well known to induce sepsis and microglial activation in the CNS. On the contrary, the effect of products from gram-positive bacteria especially in areas devoid of blood-brain barrier remains to be explored. In the present study, a panel of antibodies, namely, OX-6, OX-42 and ED-1 was used to study the response of microglia/macrophages in the pineal gland of rats given an intravenous LPS or lipoteichoic acid (LTA). These antibodies recognize MHC class II antigens, complement type 3 receptors and unknown lysosomal proteins in macrophages, respectively. In rats given LPS (50 υg/kg) injection and killed 48 h later, the cell density and immunoexpression of OX-6, OX-42 and ED-1 in pineal microglia/macrophages were markedly increased. In rats receiving a high dose (20 mg/kg) of LTA, OX-42 and OX-6, immunoreactivities in pineal microglia/macrophages were also enhanced, but that of ED-1 was not. In addition, both bacterial toxins induced an increase in astrocytic profiles labelled by glial fibrillary acid protein. An interesting feature following LPS or LTA treatment was the lowering effect on serum melatonin, enhanced serotonin immunolabelling and cellular vacuolation as studied by electron microscopy in pinealocytes. The LPS- or LTA-induced vacuoles appeared to originate from the granular endoplasmic reticulum as well as the Golgi saccules. The present results suggest that LPS and LTA could induce immune responses of microglia/macrophages and astroglial activation in the pineal gland. Furthermore, the metabolic and secretory activity of pinealocytes was modified by products from both gram-positive and -negative bacteria.

Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalJournal of Pineal Research
Volume38
Issue number1
DOIs
Publication statusPublished - Jan 2005
Externally publishedYes

Fingerprint

Pineal Gland
Gram-Positive Bacteria
Cellular Structures
Gram-Negative Bacteria
Neuroglia
Cell Wall
Lipopolysaccharides
Microglia
Macrophages
Macrophage-1 Antigen
Saccule and Utricle
Bacterial Toxins
Macrophage Activation
Antibodies
Rough Endoplasmic Reticulum
Glial Fibrillary Acidic Protein
Histocompatibility Antigens Class II
Melatonin
Vacuoles
Blood-Brain Barrier

Keywords

  • Astrocyte
  • Lipopolysaccharide
  • Lipoteichoic acid
  • Macrophage
  • Microglia
  • Pineal gland
  • Pinealocyte
  • Rat

ASJC Scopus subject areas

  • Endocrinology

Cite this

Reactive changes of interstitial glia and pinealocytes in the rat pineal gland challenged with cell wall components from gram-positive and -negative bacteria. / Jiang-Shieh, Ya Fen; Wu, Ching Hsiang; Chien, Hsiung Fei; Wei, I. Hua; Chang, Min Lin; Shieh, Jeng Yung; Wen, Chen Yuan.

In: Journal of Pineal Research, Vol. 38, No. 1, 01.2005, p. 17-26.

Research output: Contribution to journalArticle

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abstract = "Lipopolysaccharide (LPS), the major proinflammatory component of gram-negative bacteria, is well known to induce sepsis and microglial activation in the CNS. On the contrary, the effect of products from gram-positive bacteria especially in areas devoid of blood-brain barrier remains to be explored. In the present study, a panel of antibodies, namely, OX-6, OX-42 and ED-1 was used to study the response of microglia/macrophages in the pineal gland of rats given an intravenous LPS or lipoteichoic acid (LTA). These antibodies recognize MHC class II antigens, complement type 3 receptors and unknown lysosomal proteins in macrophages, respectively. In rats given LPS (50 υg/kg) injection and killed 48 h later, the cell density and immunoexpression of OX-6, OX-42 and ED-1 in pineal microglia/macrophages were markedly increased. In rats receiving a high dose (20 mg/kg) of LTA, OX-42 and OX-6, immunoreactivities in pineal microglia/macrophages were also enhanced, but that of ED-1 was not. In addition, both bacterial toxins induced an increase in astrocytic profiles labelled by glial fibrillary acid protein. An interesting feature following LPS or LTA treatment was the lowering effect on serum melatonin, enhanced serotonin immunolabelling and cellular vacuolation as studied by electron microscopy in pinealocytes. The LPS- or LTA-induced vacuoles appeared to originate from the granular endoplasmic reticulum as well as the Golgi saccules. The present results suggest that LPS and LTA could induce immune responses of microglia/macrophages and astroglial activation in the pineal gland. Furthermore, the metabolic and secretory activity of pinealocytes was modified by products from both gram-positive and -negative bacteria.",
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AU - Wei, I. Hua

AU - Chang, Min Lin

AU - Shieh, Jeng Yung

AU - Wen, Chen Yuan

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