RD-114, baboon, and woolly monkey viral RNAs compared in size and structure

Hsing Jien Kung, Sylvia Hu, Welcome Bender, James M. Bailey, Norman Davidson, Margery O. Nicolson, Robert M. McAllister

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The molecular weights, subunit compositions, and secondary structure patterns of the RNAs from an endogenous baboon virus and from a woolly monkey sarcoma virus were examined and compared to the properties of the RNA of RD-114, an endogenous feline virus. The high molecular weight RNA extracted from each of these three viruses has a sedimentation coefficient of 52S, and a molecular length, measured by electron microscopy, of 16-20 kb (kb = kilobase, 1000 nucleotides). Each such RNA is a dimer, containing two monomer subunits of 8-10 kb in length (molecular weight 3 × 106 daltons). The two monomer sub-units are joined at their non-poly(A) ends in a structure called the dimer linkage structure. The appearance of this structure is somewhat different for the different viruses. The dimer linkage dissociates at a temperature estimated to be 87°C in aqueous 0.1M Na+ for RD-114 and baboon viral RNAs, but at the lower temperature of 66°C for woolly monkey RNA. All three viral RNAs have two large loops of similar size and position symmetrically placed on either side of the dimer linkage structure. Since the baboon virus is partially related to RD-114, and the woolly monkey virus is unrelated to either of the other two, the dimer linkage and symmetrical loops are surprisingly similar and may well be common features of type C virus RNAs.

Original languageEnglish
Pages (from-to)609-620
Number of pages12
JournalCell
Volume7
Issue number4
DOIs
Publication statusPublished - Jan 1 1976
Externally publishedYes

Fingerprint

Atelinae
Papio
Viral RNA
Viruses
RNA
Woolly Monkey Sarcoma Virus
Dimers
Molecular Weight
Molecular weight
Temperature
RNA Viruses
Felidae
Monomers
Electron Microscopy
Nucleotides
Sedimentation
Electron microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kung, H. J., Hu, S., Bender, W., Bailey, J. M., Davidson, N., Nicolson, M. O., & McAllister, R. M. (1976). RD-114, baboon, and woolly monkey viral RNAs compared in size and structure. Cell, 7(4), 609-620. https://doi.org/10.1016/0092-8674(76)90211-7

RD-114, baboon, and woolly monkey viral RNAs compared in size and structure. / Kung, Hsing Jien; Hu, Sylvia; Bender, Welcome; Bailey, James M.; Davidson, Norman; Nicolson, Margery O.; McAllister, Robert M.

In: Cell, Vol. 7, No. 4, 01.01.1976, p. 609-620.

Research output: Contribution to journalArticle

Kung, HJ, Hu, S, Bender, W, Bailey, JM, Davidson, N, Nicolson, MO & McAllister, RM 1976, 'RD-114, baboon, and woolly monkey viral RNAs compared in size and structure', Cell, vol. 7, no. 4, pp. 609-620. https://doi.org/10.1016/0092-8674(76)90211-7
Kung HJ, Hu S, Bender W, Bailey JM, Davidson N, Nicolson MO et al. RD-114, baboon, and woolly monkey viral RNAs compared in size and structure. Cell. 1976 Jan 1;7(4):609-620. https://doi.org/10.1016/0092-8674(76)90211-7
Kung, Hsing Jien ; Hu, Sylvia ; Bender, Welcome ; Bailey, James M. ; Davidson, Norman ; Nicolson, Margery O. ; McAllister, Robert M. / RD-114, baboon, and woolly monkey viral RNAs compared in size and structure. In: Cell. 1976 ; Vol. 7, No. 4. pp. 609-620.
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