Rat Liver Ischemia/Reperfusion Induced Proinflammatory Mediator and Antioxidant Expressions Analyzed by Gene Chips and Real-Time Polymerase Chain Reactions

Y. C. Hsu, T. Y. Chou, C. F. Chen, D. Wang, C. L. Su, R. T. Hu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: Ischemia/reperfusion (I/R) of the rat liver induces injury; however, few studies have investigated gene expressions associated with this phenomenon. In this study, gene chip and real-time polymerase chain reactions (PCR) were used to study the expressions of the proinflammatory mediators and antioxidants after I/R. Materials and Methods: Ischemia was induced by clamping the common hepatic artery and portal vein for 40 minutes followed by 90 minutes reperfusion. Blood samples collected before ischemia and after reperfusion were analyzed for alanine amino transferase, lactic dehydrogenase, hydroxyl radicals, nitric oxide (NO), and tumor necrosis factor α (TNFα). Expressions of TNFα, interleukin 12 (IL12), cyclooxygenase II (COXII), and other inflammatory mediators were analyzed by gene chips. COXII, TNFα, and antioxidants of mitochondrial superoxide dismutase (SOD(Mn)), catalase, and heat shock protein 70 (HSP70) were double confirmed by real-time PCRs. Results: This protocol resulted in elevations in the blood concentrations of NO, hydroxyl radicals, TNFα, ALT, and LDH (P <.01) in the I/R but not the sham-operated group. Reperfusion induced significant increases in the expressions of TNFα, IL12, COXII, SOD(Mn), catalase, and HSP70. Real-time PCR also demonstrated increases in mRNA expressions of the proinflammatory mediators and antioxidants. Conclusions: This protocol resulted in oxidative stress, nitrosative stress, and liver injury. The increases in expressions of both proinflammatory mediators and antioxidants suggested that an imbalance between inflammation and anti-inflammation could be the possible reason for the liver injury after I/R.

Original languageEnglish
Pages (from-to)2156-2158
Number of pages3
JournalTransplantation Proceedings
Volume40
Issue number7
DOIs
Publication statusPublished - Sep 2008

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Oligonucleotide Array Sequence Analysis
Reperfusion
Real-Time Polymerase Chain Reaction
Ischemia
Tumor Necrosis Factor-alpha
Antioxidants
Prostaglandin-Endoperoxide Synthases
Liver
HSP70 Heat-Shock Proteins
Interleukin-12
Hydroxyl Radical
Catalase
Wounds and Injuries
Nitric Oxide
Inflammation
Hepatic Veins
Hepatic Artery
Transferases
Portal Vein
Constriction

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Rat Liver Ischemia/Reperfusion Induced Proinflammatory Mediator and Antioxidant Expressions Analyzed by Gene Chips and Real-Time Polymerase Chain Reactions. / Hsu, Y. C.; Chou, T. Y.; Chen, C. F.; Wang, D.; Su, C. L.; Hu, R. T.

In: Transplantation Proceedings, Vol. 40, No. 7, 09.2008, p. 2156-2158.

Research output: Contribution to journalArticle

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abstract = "Objective: Ischemia/reperfusion (I/R) of the rat liver induces injury; however, few studies have investigated gene expressions associated with this phenomenon. In this study, gene chip and real-time polymerase chain reactions (PCR) were used to study the expressions of the proinflammatory mediators and antioxidants after I/R. Materials and Methods: Ischemia was induced by clamping the common hepatic artery and portal vein for 40 minutes followed by 90 minutes reperfusion. Blood samples collected before ischemia and after reperfusion were analyzed for alanine amino transferase, lactic dehydrogenase, hydroxyl radicals, nitric oxide (NO), and tumor necrosis factor α (TNFα). Expressions of TNFα, interleukin 12 (IL12), cyclooxygenase II (COXII), and other inflammatory mediators were analyzed by gene chips. COXII, TNFα, and antioxidants of mitochondrial superoxide dismutase (SOD(Mn)), catalase, and heat shock protein 70 (HSP70) were double confirmed by real-time PCRs. Results: This protocol resulted in elevations in the blood concentrations of NO, hydroxyl radicals, TNFα, ALT, and LDH (P <.01) in the I/R but not the sham-operated group. Reperfusion induced significant increases in the expressions of TNFα, IL12, COXII, SOD(Mn), catalase, and HSP70. Real-time PCR also demonstrated increases in mRNA expressions of the proinflammatory mediators and antioxidants. Conclusions: This protocol resulted in oxidative stress, nitrosative stress, and liver injury. The increases in expressions of both proinflammatory mediators and antioxidants suggested that an imbalance between inflammation and anti-inflammation could be the possible reason for the liver injury after I/R.",
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AU - Su, C. L.

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