Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system

SEY EN LIN, DONG YO JHENG, KUANG YU HSU, YUN RU LIU, WEI HSIANG HUANG, HSIANG CHIEH LEE, CHIEN CHUNG TSAI

Research output: Contribution to journalArticlepeer-review

Abstract

A technique using Linnik-based optical coherence microscopy (OCM), with built-in fluorescence microscopy (FM), is demonstrated here to describe cellular-level morphology for fresh porcine and biobank tissue specimens. The proposed method utilizes color-coding to generate digital pseudo-H&E (p-H&E) images. Using the same camera, colocalized FM images are merged with corresponding morphological OCM images using a 24-bit RGB composition process to generate position-matched p-H&E images. From receipt of dissected fresh tissue piece to generation of stitched images, the total processing time is <15 min for a 1-cm2 specimen, which is on average two times faster than frozen-section H&E process for fatty or water-rich fresh tissue specimens. This technique was successfully used to scan human and animal fresh tissue pieces, demonstrating its applicability for both biobank and veterinary purposes. We provide an in-depth comparison between p-H&E and human frozen-section H&E images acquired from the same metastatic sentinel lymph node slice (∼10 μm thick), and show the differences, like elastic fibers of a tiny blood vessel and cytoplasm of tumor cells. This optical sectioning technique provides histopathologists with a convenient assessment method that outputs large-field H&E-like images of fresh tissue pieces without requiring any physical embedment.

Original languageEnglish
Pages (from-to)5139-5158
Number of pages20
JournalBiomedical Optics Express
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 1 2021

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system'. Together they form a unique fingerprint.

Cite this