Rapid molecular diagnosis of the Gilbert's syndrome-associated exon 1 mutation within the UGT1A1 gene

T. Y. Hsieh, T. Y. Shiu, N. F. Chu, T. Y. Chao, H. C. Chu, W. K. Chang, Y. C. Chao, H. H. Huang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Gilbert's syndrome is suspected in patients with unconjugated hyperbilirubinemia caused by decreased activity of the UDP-glucuronosyltransferase 1A1 (UGT1A1) gene in the absence of abnormal liver function and hemolysis. The major genetic variants underlying Gilbert's syndrome are TATA-box repeats of the promoter region and exon 1 G211A of the coding region, particularly in Asians. The efficacy of DNA melting curve analysis, however, has not been established for the G211A mutation. For rapid and accurate molecular diagnosis of Gilbert's syndrome, DNA melting curve analysis was evaluated for its genotyping capability not only for TATA-box repeats of the UGT1A1 promoter, but also for G211A of UGT1A1 exon 1. TA repeats within the TATA-box sequence and the exon 1 G211A mutation of the UGT1A1 gene were analyzed by DNA melting curve analysis. To evaluate the assay reliability, direct sequencing or polyacrylamide gel electrophoresis was used as a comparative method. All homozygous and heterozygous polymorphisms of A(TA)7TAA within the TATA-box allele and of exon 1 G211A mutants of the UGT1A1 gene were successfully identified with DNA melting curve analysis. DNA melting curve analysis is, therefore, an effective molecular method for the rapid diagnosis of Gilbert's syndrome, as it detects not only TATA-box polymorphisms but also the exon 1 G211A mutation located within the UGT1A1 gene.

Original languageEnglish
Pages (from-to)670-679
Number of pages10
JournalGenetics and Molecular Research
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 28 2014

Fingerprint

Gilbert Disease
Nucleic Acid Denaturation
TATA Box
Exons
Mutation
Genes
Hyperbilirubinemia
Hemolysis
Genetic Promoter Regions
UGT1A1 enzyme
Polyacrylamide Gel Electrophoresis
Alleles
Liver

Keywords

  • G211A mutation
  • Gilbert's syndrome
  • UDP-glucuronosyltransferase 1A1

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Rapid molecular diagnosis of the Gilbert's syndrome-associated exon 1 mutation within the UGT1A1 gene. / Hsieh, T. Y.; Shiu, T. Y.; Chu, N. F.; Chao, T. Y.; Chu, H. C.; Chang, W. K.; Chao, Y. C.; Huang, H. H.

In: Genetics and Molecular Research, Vol. 13, No. 1, 28.01.2014, p. 670-679.

Research output: Contribution to journalArticle

Hsieh, T. Y. ; Shiu, T. Y. ; Chu, N. F. ; Chao, T. Y. ; Chu, H. C. ; Chang, W. K. ; Chao, Y. C. ; Huang, H. H. / Rapid molecular diagnosis of the Gilbert's syndrome-associated exon 1 mutation within the UGT1A1 gene. In: Genetics and Molecular Research. 2014 ; Vol. 13, No. 1. pp. 670-679.
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