We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.
|Number of pages||5|
|Journal||Botanical Bulletin of Academia Sinica|
|Publication status||Published - 1998|
- Differentially expressed cDNAs
- Near isogenic line
ASJC Scopus subject areas
- Plant Science