Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin, Cheng Chun Kuan, Rong Huay Juang, Samson C S Tsou, C. George Kuo, Ching San Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalBotanical Bulletin of Academia Sinica
Volume39
Issue number2
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Differentially expressed cDNAs
  • Mungbean
  • Near isogenic line

ASJC Scopus subject areas

  • Plant Science

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  • Cite this

    Lin, C. Y., Kuan, C. C., Juang, R. H., Tsou, S. C. S., Kuo, C. G., & Chen, C. S. (1998). Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean. Botanical Bulletin of Academia Sinica, 39(2), 87-91.