Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin; Cheng Chun Kuan; Rong Huay Juang; Samson C S Tsou; C. George Kuo; Ching San Chen

Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Ching Yu Lin, Cheng Chun Kuan, Rong Huay Juang, Samson C S Tsou, C. George Kuo, Ching San Chen

Research output: Contribution to journal › Article › peer-review

Abstract

We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Botanical Bulletin of Academia Sinica
Volume	39
Issue number	2
Publication status	Published - 1998
Externally published	Yes

Keywords

Differentially expressed cDNAs
Mungbean
Near isogenic line

ASJC Scopus subject areas

Plant Science

Cite this

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title = "Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean",

abstract = "We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.",

keywords = "Differentially expressed cDNAs, Mungbean, Near isogenic line",

author = "Lin, {Ching Yu} and Kuan, {Cheng Chun} and Juang, {Rong Huay} and Tsou, {Samson C S} and Kuo, {C. George} and Chen, {Ching San}",

year = "1998",

language = "English",

volume = "39",

pages = "87--91",

journal = "Botanical Studies",

issn = "1817-406X",

publisher = "Academia Sinica",

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T1 - Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

AU - Lin, Ching Yu

AU - Kuan, Cheng Chun

AU - Juang, Rong Huay

AU - Tsou, Samson C S

AU - Kuo, C. George

AU - Chen, Ching San

PY - 1998

Y1 - 1998

N2 - We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

AB - We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1-1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.

KW - Differentially expressed cDNAs

KW - Mungbean

KW - Near isogenic line

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M3 - Article

AN - SCOPUS:0031972381

SN - 1817-406X

VL - 39

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EP - 91

JO - Botanical Studies

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IS - 2

ER -

Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean

Abstract

Keywords

ASJC Scopus subject areas

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