Rapid identification of the antioxidant constituent of Koelreuteria henryi

Chin Hui Chen, Pi Yu Chena, Kuang Chuan Wang, Ching Kuo Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In recent years, on-line coupling of separation and activity determination techniques has been achieved by mixing a free radical solution with the eluate of an HPLC column. Such techniques enable the rapid and selective detection of radical scavenging substances in the sample preparation. This study investigates the antioxidant properties of the leaves of Koelreuteria henryi L. using a microarray reader and HPLC. The antioxidant, 1,3,4,5-tetra-O-galloylquinic acid (1), was first isolated from this plant and estimated using LC-MS/MS and NMR methods. The scavenger activity of (1) was then determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine oxidase (XOD) assays, producing an IC50 of 7.1 μM and 6.7 μM, respectively. Results show that this compound has an excellent antioxidant effect in comparison with quercetin. This study shows that it is possible to rapidly identify the antioxidant constituents of K. henryi L. using the hyphenated DPPH (or XOD) assays and LC-MS/MS methods.

Original languageEnglish
Pages (from-to)404-410
Number of pages7
JournalJournal of the Chinese Chemical Society
Volume57
Issue number3 A
Publication statusPublished - 2010

Keywords

  • DPPH
  • Koelreuteria henryi
  • LC-MS/MS
  • Xanthine oxidase

ASJC Scopus subject areas

  • Chemistry(all)

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