Rapid diagnosis of tuberculous meningitis by polymerase chain reaction assay of cerebrospinal fluid

Juei Jueng Lin, Horng Jyh Harn, Yaw Don Hsu, Wen Long Tsao, Herng Sheng Lee, Wei Hwa Lee

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) method for the rapid diagnosis of tuberculous meningitis (TBM) was used to study prospectively 47 cerebrospinal fluid (CSF) samples from 45 patients. Twenty CSF samples were from patients with clinically suspected TBM and another 27 samples came from patients without clinically suspected TBM. Mycobacterial DNA was detected in 15 CSF samples (14 from patients with clinically suspected TBM and 1 from a patient not suspected of having TBM). Of the PCR-positive samples, 4 were also positive for mycobacterial culture. However, 32 PCR-negative samples were all culture-negative. All samples were negative for the acid-fast bacillus by direct smear. The single PCR-positive patient in the clinically unsuspected TBM group was initially diagnosed as suffering from aseptic meningitis on the basis of his clinical features. The mycobacterial culture of his CSF specimen was also positive and a revised diagnosis of an aseptic type of TBM was made. The estimations of specificity and sensitivity in this study were 100% and 70% respectively. The results showed that using a PCR to detect mycobacterial DNA in CSF for the early diagnosis of TBM is not only a rapid but also an accurate method.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalJournal of Neurology
Volume242
Issue number3
DOIs
Publication statusPublished - Mar 1995
Externally publishedYes

Fingerprint

Meningeal Tuberculosis
Cerebrospinal Fluid
Polymerase Chain Reaction
Aseptic Meningitis
DNA
Bacillus
Early Diagnosis
Sensitivity and Specificity
Acids

Keywords

  • Deoxyribonucleic acid
  • Mycobacterium tuberculosis
  • Polymerase chain reaction
  • Tuberculous meningitis

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Rapid diagnosis of tuberculous meningitis by polymerase chain reaction assay of cerebrospinal fluid. / Lin, Juei Jueng; Harn, Horng Jyh; Hsu, Yaw Don; Tsao, Wen Long; Lee, Herng Sheng; Lee, Wei Hwa.

In: Journal of Neurology, Vol. 242, No. 3, 03.1995, p. 147-152.

Research output: Contribution to journalArticle

Lin, Juei Jueng ; Harn, Horng Jyh ; Hsu, Yaw Don ; Tsao, Wen Long ; Lee, Herng Sheng ; Lee, Wei Hwa. / Rapid diagnosis of tuberculous meningitis by polymerase chain reaction assay of cerebrospinal fluid. In: Journal of Neurology. 1995 ; Vol. 242, No. 3. pp. 147-152.
@article{8e244fdc38634a3f88af3cc46d5c5833,
title = "Rapid diagnosis of tuberculous meningitis by polymerase chain reaction assay of cerebrospinal fluid",
abstract = "A polymerase chain reaction (PCR) method for the rapid diagnosis of tuberculous meningitis (TBM) was used to study prospectively 47 cerebrospinal fluid (CSF) samples from 45 patients. Twenty CSF samples were from patients with clinically suspected TBM and another 27 samples came from patients without clinically suspected TBM. Mycobacterial DNA was detected in 15 CSF samples (14 from patients with clinically suspected TBM and 1 from a patient not suspected of having TBM). Of the PCR-positive samples, 4 were also positive for mycobacterial culture. However, 32 PCR-negative samples were all culture-negative. All samples were negative for the acid-fast bacillus by direct smear. The single PCR-positive patient in the clinically unsuspected TBM group was initially diagnosed as suffering from aseptic meningitis on the basis of his clinical features. The mycobacterial culture of his CSF specimen was also positive and a revised diagnosis of an aseptic type of TBM was made. The estimations of specificity and sensitivity in this study were 100{\%} and 70{\%} respectively. The results showed that using a PCR to detect mycobacterial DNA in CSF for the early diagnosis of TBM is not only a rapid but also an accurate method.",
keywords = "Deoxyribonucleic acid, Mycobacterium tuberculosis, Polymerase chain reaction, Tuberculous meningitis",
author = "Lin, {Juei Jueng} and Harn, {Horng Jyh} and Hsu, {Yaw Don} and Tsao, {Wen Long} and Lee, {Herng Sheng} and Lee, {Wei Hwa}",
year = "1995",
month = "3",
doi = "10.1007/BF00936887",
language = "English",
volume = "242",
pages = "147--152",
journal = "Journal of Neurology",
issn = "0340-5354",
publisher = "D. Steinkopff-Verlag",
number = "3",

}

TY - JOUR

T1 - Rapid diagnosis of tuberculous meningitis by polymerase chain reaction assay of cerebrospinal fluid

AU - Lin, Juei Jueng

AU - Harn, Horng Jyh

AU - Hsu, Yaw Don

AU - Tsao, Wen Long

AU - Lee, Herng Sheng

AU - Lee, Wei Hwa

PY - 1995/3

Y1 - 1995/3

N2 - A polymerase chain reaction (PCR) method for the rapid diagnosis of tuberculous meningitis (TBM) was used to study prospectively 47 cerebrospinal fluid (CSF) samples from 45 patients. Twenty CSF samples were from patients with clinically suspected TBM and another 27 samples came from patients without clinically suspected TBM. Mycobacterial DNA was detected in 15 CSF samples (14 from patients with clinically suspected TBM and 1 from a patient not suspected of having TBM). Of the PCR-positive samples, 4 were also positive for mycobacterial culture. However, 32 PCR-negative samples were all culture-negative. All samples were negative for the acid-fast bacillus by direct smear. The single PCR-positive patient in the clinically unsuspected TBM group was initially diagnosed as suffering from aseptic meningitis on the basis of his clinical features. The mycobacterial culture of his CSF specimen was also positive and a revised diagnosis of an aseptic type of TBM was made. The estimations of specificity and sensitivity in this study were 100% and 70% respectively. The results showed that using a PCR to detect mycobacterial DNA in CSF for the early diagnosis of TBM is not only a rapid but also an accurate method.

AB - A polymerase chain reaction (PCR) method for the rapid diagnosis of tuberculous meningitis (TBM) was used to study prospectively 47 cerebrospinal fluid (CSF) samples from 45 patients. Twenty CSF samples were from patients with clinically suspected TBM and another 27 samples came from patients without clinically suspected TBM. Mycobacterial DNA was detected in 15 CSF samples (14 from patients with clinically suspected TBM and 1 from a patient not suspected of having TBM). Of the PCR-positive samples, 4 were also positive for mycobacterial culture. However, 32 PCR-negative samples were all culture-negative. All samples were negative for the acid-fast bacillus by direct smear. The single PCR-positive patient in the clinically unsuspected TBM group was initially diagnosed as suffering from aseptic meningitis on the basis of his clinical features. The mycobacterial culture of his CSF specimen was also positive and a revised diagnosis of an aseptic type of TBM was made. The estimations of specificity and sensitivity in this study were 100% and 70% respectively. The results showed that using a PCR to detect mycobacterial DNA in CSF for the early diagnosis of TBM is not only a rapid but also an accurate method.

KW - Deoxyribonucleic acid

KW - Mycobacterium tuberculosis

KW - Polymerase chain reaction

KW - Tuberculous meningitis

UR - http://www.scopus.com/inward/record.url?scp=0028817564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028817564&partnerID=8YFLogxK

U2 - 10.1007/BF00936887

DO - 10.1007/BF00936887

M3 - Article

VL - 242

SP - 147

EP - 152

JO - Journal of Neurology

JF - Journal of Neurology

SN - 0340-5354

IS - 3

ER -