Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR)

J. G. Chang, C. P. Chang, C. M. Lu, J. M. Huang, J. T. Chen, H. J. Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The detection of molecular defects of hemoglobin variants using mutagenically separated polymerase chain reaction (MS-PCR) was applied in this study. Using different lengths of allele-specific mutagenic primers, normal and mutant alleles of hemoglobin genes were amplified in the same reaction tube. Subsequent gel electrophoresis showed at least one of the two allelic products at the same loci or at least two of the several allelic products at different loci. We employed MS-PCR to test the following hemoglobin variants: Hb Constant Spring (Hb CS), Hb E, Hb G-Taichung, Hb J-Meinung, and Hb Kaohsiung. The results were the same as those obtained by amplified created reaction sites (ACRS) or direct sequencing. We conclude that the MS-PCR provides a rapid and simple alternative to other techniques for mutation detection in hemoglobin variants. Moreover, the principle can be extended to other genetic diseases.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalAnnals of Hematology
Volume71
Issue number2
DOIs
Publication statusPublished - Aug 1995
Externally publishedYes

Fingerprint

Hemoglobins
Polymerase Chain Reaction
Alleles
Inborn Genetic Diseases
Electrophoresis
Gels
Mutation
Genes

Keywords

  • Allele-specific primer
  • Hemoglobin variant
  • MS-PCR

ASJC Scopus subject areas

  • Hematology

Cite this

Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR). / Chang, J. G.; Chang, C. P.; Lu, C. M.; Huang, J. M.; Chen, J. T.; Liu, H. J.

In: Annals of Hematology, Vol. 71, No. 2, 08.1995, p. 97-100.

Research output: Contribution to journalArticle

Chang, J. G. ; Chang, C. P. ; Lu, C. M. ; Huang, J. M. ; Chen, J. T. ; Liu, H. J. / Rapid detection of hemoglobin variants by mutagenically separate polymerase chain reaction (MS-PCR). In: Annals of Hematology. 1995 ; Vol. 71, No. 2. pp. 97-100.
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