Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.

L. J. Teng, S. W. Ho, H. N. Ho, S. J. Liaw, H. C. Lai, Kwen Tay Luh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.

Original languageEnglish
Pages (from-to)396-400
Number of pages5
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume94
Issue number7
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Molecular Sequence Data
Bacterial Typing Techniques
Ureaplasma urealyticum
Polymerase Chain Reaction
Ureaplasma
Antigens
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR. / Teng, L. J.; Ho, S. W.; Ho, H. N.; Liaw, S. J.; Lai, H. C.; Luh, Kwen Tay.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 94, No. 7, 01.01.1995, p. 396-400.

Research output: Contribution to journalArticle

@article{784f695105994599a0ea2167b689f9aa,
title = "Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.",
abstract = "On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95{\%}. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.",
author = "Teng, {L. J.} and Ho, {S. W.} and Ho, {H. N.} and Liaw, {S. J.} and Lai, {H. C.} and Luh, {Kwen Tay}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "94",
pages = "396--400",
journal = "Journal of the Formosan Medical Association",
issn = "0929-6646",
publisher = "Elsevier Science Publishers B.V.",
number = "7",

}

TY - JOUR

T1 - Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.

AU - Teng, L. J.

AU - Ho, S. W.

AU - Ho, H. N.

AU - Liaw, S. J.

AU - Lai, H. C.

AU - Luh, Kwen Tay

PY - 1995/1/1

Y1 - 1995/1/1

N2 - On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.

AB - On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.

UR - http://www.scopus.com/inward/record.url?scp=0029340159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029340159&partnerID=8YFLogxK

M3 - Article

VL - 94

SP - 396

EP - 400

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

IS - 7

ER -