Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy

Hsing Yuan Li, Shing Hua Tseng, Tsai Mu Cheng, Hsueh Liang Chu, Yu Ning Lu, Fang Yu Wang, Li Yun Tsai, Juo Yu Shieh, Jyh Yuan Yang, Chien Chang Juan, Lung Chen Tu, Chia Ching Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 μl reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.

Original languageEnglish
Article number285102
JournalNanotechnology
Volume24
Issue number28
DOIs
Publication statusPublished - Jul 19 2013

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)
  • Medicine(all)

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  • Cite this

    Li, H. Y., Tseng, S. H., Cheng, T. M., Chu, H. L., Lu, Y. N., Wang, F. Y., Tsai, L. Y., Shieh, J. Y., Yang, J. Y., Juan, C. C., Tu, L. C., & Chang, C. C. (2013). Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy. Nanotechnology, 24(28), [285102]. https://doi.org/10.1088/0957-4484/24/28/285102