Rapid activation of Stat3 and ERK1/2 by nicotine modulates cell proliferation in human bladder cancer cells

Rong Jane Chen, Yuan Soon Ho, How Ran Guo, Ying Jan Wang

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Cigarette smoke is a major risk factor for bladder cancer. The main component in cigarette smoke, nicotine, can be detected in the urine of smokers. Nicotine has been implicated as a cocarcinogen that promotes lung cancer development through prosurvival pathways. Although the mechanisms of nicotine-induced cell proliferation have been well studied in lung epithelial cells, the molecular mechanism of its action in bladder epithelial cells is still unclear. The aims of this study were to investigate whether there is nicotine-induced bladder epithelial cell proliferation and to identify the signaling transduction pathway regulated by nicotine. We found that nicotine simultaneously activates Stat3 and extracellular signal regulated kinase 1/2 (ERK1/2) in T24 cells. Stat3 activation via nicotinic acetylcholine receptor (nAChR)/ protein kinase C signaling pathway was closely linked to Stat3 induction and nuclear factor-κB DNA binding activity, which is associated with Cyclin D1 expression and cell proliferation. ERK1/2 activation through nAChR and β-adrenoceptors plays a dual role in cell proliferation; it phosphorylates Stat3 at Ser727 and regulates cell proliferation. We conclude that through nAChR and β-adrenoceptors, nicotine activates ERK1/2 and Stat3 signaling pathways, leading to Cyclin D1 expression and cell proliferation. This is the first study to investigate signaling effects of nicotine in bladder cells. The current findings suggest that people exposed to nicotine could be at risk for potential deleterious effects, including bladder cancer development.

Original languageEnglish
Pages (from-to)283-293
Number of pages11
JournalToxicological Sciences
Volume104
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Cell proliferation
Nicotine
Urinary Bladder Neoplasms
Chemical activation
Cells
Cell Proliferation
Nicotinic Receptors
Urinary Bladder
Epithelial Cells
Cyclin D1
Smoke
Tobacco Products
Adrenergic Receptors
Protein Kinase C
Lung Neoplasms
Urine
Lung
DNA

Keywords

  • Bladder cancer
  • ERK1/2
  • Nicotine
  • Nicotinic acetylcholine receptor
  • Stat3

ASJC Scopus subject areas

  • Toxicology

Cite this

Rapid activation of Stat3 and ERK1/2 by nicotine modulates cell proliferation in human bladder cancer cells. / Chen, Rong Jane; Ho, Yuan Soon; Guo, How Ran; Wang, Ying Jan.

In: Toxicological Sciences, Vol. 104, No. 2, 08.2008, p. 283-293.

Research output: Contribution to journalArticle

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