RAPD analysis of Angelica sinensis medicine in Taiwan market

Kur Ta Cheng, Chia Ping June, Hsien Chang Chang

Research output: Contribution to journalArticle

Abstract

In the study, three species of Angelicae Radix, Angelica sinensis, A. acutiloba, A. gigas, and A. pubescens, a confused material with the former, were analysis using RAPD technique. Sixty oligonucleotide primers were also used to screen twenty randomly selected samples sold by the stores in the analysis. Total DNA extracted from the root of the medicine material was used as template in the PCR reaction. The RAPD pattern of OPG-15 revealed polymorphism among the different species samples, and the samples bought from the stores were demonstrated to be A. sinensis. In addition, two primers, OPD-05 and OPD-17, showed distinct two types among the samples collected from the stores, while others exhibit almost the same profiles.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalChinese Pharmaceutical Journal
Volume51
Issue number5
Publication statusPublished - Oct 1999

Fingerprint

Angelica sinensis
Taiwan
Medicine
Random Amplified Polymorphic DNA Technique
DNA Primers
Oligonucleotides
Polymorphism
DNA
Polymerase Chain Reaction

Keywords

  • Angelica sinensis
  • Chinese medicine
  • RAPD

ASJC Scopus subject areas

  • Bioengineering
  • Pharmaceutical Science

Cite this

RAPD analysis of Angelica sinensis medicine in Taiwan market. / Cheng, Kur Ta; June, Chia Ping; Chang, Hsien Chang.

In: Chinese Pharmaceutical Journal, Vol. 51, No. 5, 10.1999, p. 307-312.

Research output: Contribution to journalArticle

Cheng, Kur Ta ; June, Chia Ping ; Chang, Hsien Chang. / RAPD analysis of Angelica sinensis medicine in Taiwan market. In: Chinese Pharmaceutical Journal. 1999 ; Vol. 51, No. 5. pp. 307-312.
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