Radix Paeoniae Rubra stimulates osteoclast differentiation by activation of the NF-ΚB and mitogen-activated protein kinase pathways

Huey En Tzeng, Chun Hao Tsai, Tin Yun Ho, Chin Tung Hsieh, Shen Chieh Chou, Yi Ju Lee, Gregory J. Tsay, Po Hao Huang, Yi Ying Wu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Radix Paeoniae Rubra (RPR), a traditional Chinese herb, has anti-inflammatory and immuno-regulatory properties. This study explored the effects of RPR on stimulation of osteoclast differentiation in RAW264.7 cells and peripheral blood mononuclear cells (PBMC)s. Methods: The mature osteoclasts were measured by bone resorption assays and TRAP staining. JNK, ERK, p38 and NF-ΚB inhibitors were used applied in order to verify their contribution in RPR-induced osteoclast differentiation. The NF-ΚB and MAPK pathways were evaluated by western blotting, RT-PCR and luciferase assay. Results: RPR induced osteoclast differentiation in a dose-dependent manner and induced the resorption activity of osteoclasts differentiation of RAW264.7 cells and PBMCs. Western blotting showed that RPR treatment induced phosphorylation of JNK, ERK, and p38 in RAW 264.7 cells. Treatment of JNK, ERK, and p38 MAP kinase inhibitors verified the contribution of JNK, ERK and p38. RPR treatment induced c-Fos and NFATc1 protein expression; NF-ΚB inhibitor treatment and luciferase assay verified the contribution of the NF-ΚB pathway. Conclusions: This study demonstrated the interesting effect, in which RPR stimulated osteoclast differentiation in murine RAW264.7 cells and human monocytes.

Original languageEnglish
Article number132
JournalBMC Complementary and Alternative Medicine
Volume18
Issue number1
DOIs
Publication statusPublished - Apr 23 2018

Keywords

  • Mitogen-activated protein kinase
  • Osteoclast
  • Receptor activator of nuclear factor-kappa B

ASJC Scopus subject areas

  • Complementary and alternative medicine

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