Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells

Hsin Yi Chang, Ming Hua Li, Tsui Chin Huang, Chia Lang Hsu, Shang Ru Tsai, Si Chen Lee, Hsuan Cheng Huang, Hsueh Fen Juan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Breast cancer is one of the leading cancer-related causes of death worldwide. Treatment of triple-negative breast cancer (TNBC) is complex and challenging, especially when metastasis has developed. In this study, we applied infrared radiation as an alternative approach for the treatment of TNBC. We used middle infrared (MIR) with a wavelength range of 3-5 Îm to irradiate breast cancer cells. MIR significantly inhibited cell proliferation in several breast cancer cells but did not affect the growth of normal breast epithelial cells. We performed iTRAQ-coupled LC-MS/MS analysis to investigate the MIR-triggered molecular mechanisms in breast cancer cells. A total of 1749 proteins were identified, quantified, and subjected to functional enrichment analysis. From the constructed functionally enriched network, we confirmed that MIR caused G2/M cell cycle arrest, remodeled the microtubule network to an astral pole arrangement, altered the actin filament formation and focal adhesion molecule localization, and reduced cell migration activity and invasion ability. Our results reveal the coordinative effects of MIR-regulated physiological responses in concentrated networks, demonstrating the potential implementation of infrared radiation in breast cancer therapy.

Original languageEnglish
Pages (from-to)1250-1262
Number of pages13
JournalJournal of Proteome Research
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 6 2015

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Proteomics
Cells
Radiation
Breast Neoplasms
Infrared radiation
Triple Negative Breast Neoplasms
G2 Phase Cell Cycle Checkpoints
Focal Adhesions
Actin Cytoskeleton
Microtubules
Cell Movement
Cause of Death
Breast
Cell proliferation
Epithelial Cells
Cell Proliferation
Neoplasm Metastasis
Actins
Poles
Adhesion

Keywords

  • cell cycle arrest
  • cytoskeleton remodeling
  • infrared radiation
  • iTRAQ
  • triple negative breast cancer

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells. / Chang, Hsin Yi; Li, Ming Hua; Huang, Tsui Chin; Hsu, Chia Lang; Tsai, Shang Ru; Lee, Si Chen; Huang, Hsuan Cheng; Juan, Hsueh Fen.

In: Journal of Proteome Research, Vol. 14, No. 2, 06.02.2015, p. 1250-1262.

Research output: Contribution to journalArticle

Chang, Hsin Yi ; Li, Ming Hua ; Huang, Tsui Chin ; Hsu, Chia Lang ; Tsai, Shang Ru ; Lee, Si Chen ; Huang, Hsuan Cheng ; Juan, Hsueh Fen. / Quantitative proteomics reveals middle infrared radiation-interfered networks in breast cancer cells. In: Journal of Proteome Research. 2015 ; Vol. 14, No. 2. pp. 1250-1262.
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