Quantitative of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ming Hui Yang, Han Ping You, Hsin Yi Wu, Yi Ming Arthur Chen, Ying Fong Huang, Yu Chang Tyan

Research output: Contribution to journalArticlepeer-review

Abstract

A quantification assay based on isotope dilution mass spectrometry to determine the concentration of progesterone in human serum was reported. Incorporated with 13C3-progesterone, serum samples were subjected to progesterone extraction and clean-up by C4 solid-phase-extraction columns and hexane-based liquid/liquid extraction, respectively. The cleaned-up serum samples were then subjected to MALDI-TOF mass spectrometry for the quantification of progesterone. In the study, the recovered progesterone concentration determined by the assay showed good robustness and constancy in comparison to conventional radioimmunologic assay. We concluded that the 13C3-progesterone-based quantification assay is a robust method for the measurement of serum progesterone. Advantages of this technique includes: • This study describes a MALDI-TOF/MS method for the determination of serum progesterone. • The technique is simple and easy to apply on MALDI-TOF/MS for serum progesterone analysis. • The correlation coefficient between MALDI-TOF MS and RIA was 0.981 for serum progesterone.
Original languageEnglish
Article number100812
Pages (from-to)100812
JournalMethodsX
Volume7
DOIs
Publication statusPublished - Jan 1 2020

Keywords

  • Isotope dilution
  • MALDI-TOF/MS
  • Progesterone
  • Quantitative of progesterone

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology

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