Quantitative MR T2 measurement of articular cartilage to assess the treatment effect of intra-articular hyaluronic acid injection on experimental osteoarthritis induced by ACLX

G. S. Huang, H. S. Lee, M. C. Chou, Y. Y I Shih, P. H. Tsai, M. H. Lin, C. Y. Lin, C. H. Lee, H. W. Chung

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Abstract

Objective: The purpose of this study was to investigate whether the effect of treatment with hyaluronic acid (HA) on cartilage in osteoarthritis (OA) can be determined by measuring the magnetic resonance (MR) T2 value of cartilage in an anterior cruciate ligament transection (ACLX) animal model. Method: Eighteen male Sprague Dawley rats were separated randomly into three groups (n = 6 for each group). Group 1 was given ACLX and intra-articular (IA) normal saline (NS) injection (ACLX + NS), group 2 was given ACLX and IA HA injection (ACLX + HA), and group 3 was the sham control. The ACLX + NS and ACLX + HA groups received ACLX on the right knee at 8 weeks of age and were then treated with IA NS or HA injection once a week, respectively, for 4 weeks starting at 13 weeks of age. In the sham-control group, the right knee joint was opened surgically but ACLX was not performed at 8 weeks of age. MR T2 measurements were obtained on all rats at 8, 12, and 21 weeks of age, and histological Mankin scoring was performed at 21 weeks of age. Results: Five weeks after the 4-week treatment, the MR T2 value of the ACLX right knee cartilage was significantly lower in ACLX + HA (29.58 ± 1.12 ms) than in ACLX + NS (32.04 ± 1.39 ms) (P <0.05). Five weeks after the 4-week treatment, the Mankin score of the ACLX right knee was significantly lower in ACLX + HA (3.3 ± 0.81) than in ACLX + NS (7.3 ± 1.03) (P <0.001). The T2 value was significantly and positively correlated with the Mankin score in the ACLX + NS (ρ = 0.77, P <0.05) and ACLX + HA (ρ = 0.69, P <0.05) groups. Conclusion: This study demonstrates the feasibility of quantitative MR T2 measurement in the early assessment of HA treatment efficiency in a cartilage degeneration model.

Original languageEnglish
Pages (from-to)54-60
Number of pages7
JournalOsteoarthritis and Cartilage
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 2010
Externally publishedYes

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Magnetic resonance measurement
Hyaluronic acid
Cartilage
Articular Cartilage
Hyaluronic Acid
Osteoarthritis
Magnetic Resonance Spectroscopy
Joints
Injections
Knee
Therapeutics
Magnetic resonance
Rats
Anterior Cruciate Ligament
Ligaments
Feasibility Studies
Knee Joint
Sprague Dawley Rats
Animals
Animal Models

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Quantitative MR T2 measurement of articular cartilage to assess the treatment effect of intra-articular hyaluronic acid injection on experimental osteoarthritis induced by ACLX. / Huang, G. S.; Lee, H. S.; Chou, M. C.; Shih, Y. Y I; Tsai, P. H.; Lin, M. H.; Lin, C. Y.; Lee, C. H.; Chung, H. W.

In: Osteoarthritis and Cartilage, Vol. 18, No. 1, 01.2010, p. 54-60.

Research output: Contribution to journalArticle

Huang, G. S. ; Lee, H. S. ; Chou, M. C. ; Shih, Y. Y I ; Tsai, P. H. ; Lin, M. H. ; Lin, C. Y. ; Lee, C. H. ; Chung, H. W. / Quantitative MR T2 measurement of articular cartilage to assess the treatment effect of intra-articular hyaluronic acid injection on experimental osteoarthritis induced by ACLX. In: Osteoarthritis and Cartilage. 2010 ; Vol. 18, No. 1. pp. 54-60.
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abstract = "Objective: The purpose of this study was to investigate whether the effect of treatment with hyaluronic acid (HA) on cartilage in osteoarthritis (OA) can be determined by measuring the magnetic resonance (MR) T2 value of cartilage in an anterior cruciate ligament transection (ACLX) animal model. Method: Eighteen male Sprague Dawley rats were separated randomly into three groups (n = 6 for each group). Group 1 was given ACLX and intra-articular (IA) normal saline (NS) injection (ACLX + NS), group 2 was given ACLX and IA HA injection (ACLX + HA), and group 3 was the sham control. The ACLX + NS and ACLX + HA groups received ACLX on the right knee at 8 weeks of age and were then treated with IA NS or HA injection once a week, respectively, for 4 weeks starting at 13 weeks of age. In the sham-control group, the right knee joint was opened surgically but ACLX was not performed at 8 weeks of age. MR T2 measurements were obtained on all rats at 8, 12, and 21 weeks of age, and histological Mankin scoring was performed at 21 weeks of age. Results: Five weeks after the 4-week treatment, the MR T2 value of the ACLX right knee cartilage was significantly lower in ACLX + HA (29.58 ± 1.12 ms) than in ACLX + NS (32.04 ± 1.39 ms) (P <0.05). Five weeks after the 4-week treatment, the Mankin score of the ACLX right knee was significantly lower in ACLX + HA (3.3 ± 0.81) than in ACLX + NS (7.3 ± 1.03) (P <0.001). The T2 value was significantly and positively correlated with the Mankin score in the ACLX + NS (ρ = 0.77, P <0.05) and ACLX + HA (ρ = 0.69, P <0.05) groups. Conclusion: This study demonstrates the feasibility of quantitative MR T2 measurement in the early assessment of HA treatment efficiency in a cartilage degeneration model.",
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AU - Lee, H. S.

AU - Chou, M. C.

AU - Shih, Y. Y I

AU - Tsai, P. H.

AU - Lin, M. H.

AU - Lin, C. Y.

AU - Lee, C. H.

AU - Chung, H. W.

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N2 - Objective: The purpose of this study was to investigate whether the effect of treatment with hyaluronic acid (HA) on cartilage in osteoarthritis (OA) can be determined by measuring the magnetic resonance (MR) T2 value of cartilage in an anterior cruciate ligament transection (ACLX) animal model. Method: Eighteen male Sprague Dawley rats were separated randomly into three groups (n = 6 for each group). Group 1 was given ACLX and intra-articular (IA) normal saline (NS) injection (ACLX + NS), group 2 was given ACLX and IA HA injection (ACLX + HA), and group 3 was the sham control. The ACLX + NS and ACLX + HA groups received ACLX on the right knee at 8 weeks of age and were then treated with IA NS or HA injection once a week, respectively, for 4 weeks starting at 13 weeks of age. In the sham-control group, the right knee joint was opened surgically but ACLX was not performed at 8 weeks of age. MR T2 measurements were obtained on all rats at 8, 12, and 21 weeks of age, and histological Mankin scoring was performed at 21 weeks of age. Results: Five weeks after the 4-week treatment, the MR T2 value of the ACLX right knee cartilage was significantly lower in ACLX + HA (29.58 ± 1.12 ms) than in ACLX + NS (32.04 ± 1.39 ms) (P <0.05). Five weeks after the 4-week treatment, the Mankin score of the ACLX right knee was significantly lower in ACLX + HA (3.3 ± 0.81) than in ACLX + NS (7.3 ± 1.03) (P <0.001). The T2 value was significantly and positively correlated with the Mankin score in the ACLX + NS (ρ = 0.77, P <0.05) and ACLX + HA (ρ = 0.69, P <0.05) groups. Conclusion: This study demonstrates the feasibility of quantitative MR T2 measurement in the early assessment of HA treatment efficiency in a cartilage degeneration model.

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