Quantitative determination of isoniazid in biological samples by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography

I. Lin Tsai, Hsiang Yin Liu, Ping Hung Kuo, Jann Yuan Wang, Li Jiuan Shen, Ching Hua Kuo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, infects approximately one third of the current world population. Isoniazid is one of the most frequently used first-line anti-TB drugs. In this study, we developed a sensitive cation-selective exhaustive injection-sweeping- micellar electrokinetic chromatography method (CSEI-Sweep-MEKC) for analyzing isoniazid in human plasma. Parameters including acetonitrile (ACN) percentage in the separation buffer; the injection time, and concentration of the high-conductivity buffer; sodium dodecyl sulfate (SDS) concentration; phosphate concentration in the sample matrix; and the sample injection time were all optimized to obtain the best analytical performance. The optimal background electrolyte comprised 50 mM phosphate buffer, 100 mM SDS, and 15% ACN. Non-micelle background electrolyte, containing 75 mM phosphate buffer and 15% ACN, was first injected into the capillary, followed by a short plug of 200 mM phosphate (high-conductivity buffer). Run-to-run repeatability (n∈=∈3) and intermediate precision (n∈=∈3) of peak area ratios were found to be lower than 8.7% and 11.4% RSD, respectively. The accuracy of the method was within 98.1-106.9%. The limit of detection of isoniazod in human plasma was 9 ng mL -1. Compared with conventional MEKC, the enhancement factor of the CSEI-Sweep-MEKC method was 85 in plasma samples. The developed method was successfully used to determine isoniazid concentration in patient plasma. The results demonstrated that CSEI-Sweep-MEKC has the potential to analyze isoniazid in human plasma for therapeutic drug monitoring and clinical research.

Original languageEnglish
Pages (from-to)2205-2214
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume401
Issue number7
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Isoniazid
Chromatography
Cations
Buffers
Plasma (human)
Injections
Phosphates
Sodium Dodecyl Sulfate
Electrolytes
Tuberculosis
Plasmas
Micellar Electrokinetic Capillary Chromatography
Drug Monitoring
Pharmaceutical Preparations
Mycobacterium tuberculosis
Communicable Diseases
Limit of Detection
Monitoring
acetonitrile
Research

Keywords

  • Cation-selective exhaustive injection
  • Human plasma
  • Isoniazid
  • Sweeping

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Quantitative determination of isoniazid in biological samples by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography. / Tsai, I. Lin; Liu, Hsiang Yin; Kuo, Ping Hung; Wang, Jann Yuan; Shen, Li Jiuan; Kuo, Ching Hua.

In: Analytical and Bioanalytical Chemistry, Vol. 401, No. 7, 10.2011, p. 2205-2214.

Research output: Contribution to journalArticle

Tsai, I. Lin ; Liu, Hsiang Yin ; Kuo, Ping Hung ; Wang, Jann Yuan ; Shen, Li Jiuan ; Kuo, Ching Hua. / Quantitative determination of isoniazid in biological samples by cation-selective exhaustive injection-sweeping-micellar electrokinetic chromatography. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 401, No. 7. pp. 2205-2214.
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AU - Tsai, I. Lin

AU - Liu, Hsiang Yin

AU - Kuo, Ping Hung

AU - Wang, Jann Yuan

AU - Shen, Li Jiuan

AU - Kuo, Ching Hua

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AB - Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, infects approximately one third of the current world population. Isoniazid is one of the most frequently used first-line anti-TB drugs. In this study, we developed a sensitive cation-selective exhaustive injection-sweeping- micellar electrokinetic chromatography method (CSEI-Sweep-MEKC) for analyzing isoniazid in human plasma. Parameters including acetonitrile (ACN) percentage in the separation buffer; the injection time, and concentration of the high-conductivity buffer; sodium dodecyl sulfate (SDS) concentration; phosphate concentration in the sample matrix; and the sample injection time were all optimized to obtain the best analytical performance. The optimal background electrolyte comprised 50 mM phosphate buffer, 100 mM SDS, and 15% ACN. Non-micelle background electrolyte, containing 75 mM phosphate buffer and 15% ACN, was first injected into the capillary, followed by a short plug of 200 mM phosphate (high-conductivity buffer). Run-to-run repeatability (n∈=∈3) and intermediate precision (n∈=∈3) of peak area ratios were found to be lower than 8.7% and 11.4% RSD, respectively. The accuracy of the method was within 98.1-106.9%. The limit of detection of isoniazod in human plasma was 9 ng mL -1. Compared with conventional MEKC, the enhancement factor of the CSEI-Sweep-MEKC method was 85 in plasma samples. The developed method was successfully used to determine isoniazid concentration in patient plasma. The results demonstrated that CSEI-Sweep-MEKC has the potential to analyze isoniazid in human plasma for therapeutic drug monitoring and clinical research.

KW - Cation-selective exhaustive injection

KW - Human plasma

KW - Isoniazid

KW - Sweeping

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