Quantitative determination of a novel pseudoalkaloid colchatetralene isolated from the mycoflora of Gloriosa superba Linn by HPTLC

Abhishek Budhiraja, Kunal Nepali, Vinod Gouttam, Sameer Sapra, Kanaya Lal Dhar

Research output: Contribution to journalArticle

Abstract

A High performance thin layer chromatography (HPTLC) method was developed and optimized for determination of colchatetralene, a new bioactive alkaloid isolated from the chloroform extract of an endophytic fungus, isolated from surface sterilized seeds of Gloriosa superba Linn. The development of method involved evaluation and optimization of the various stages of sample preparation, chromatographic separation, detection and quantification. The method employed aluminum backed pre coated TLC plates (silica gel 60F254) as the stationary phase. The mobile phase system consisted of ethyl acetate-methanol-water (10:1.5:1, v/v/v) at room temperature (25 ± 2°C). Densitometric analysis was carried out in the absorbance/reflectance mode at the wavelength of 236nm. The system was found to give compact spots for colchatetralene (Rf value of 0.36 ± 0.03). The linear regression analysis data for the calibration plots showed good linearity (r2 = 0.99601 ± 0.002) in the concentration range 200–1200 ng spot-1. The method was validated according to the International Conference on Harmonization (ICH) guidelines for precision, accuracy, recovery and robustness. The limits of detection and quantification were 20 and 60 ng per spot, respectively.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalNatural Products Journal
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes

Keywords

  • Colchatetralene
  • Endophyte
  • Gloriosa superba
  • HPTLC
  • Validation

ASJC Scopus subject areas

  • Drug Discovery
  • Complementary and alternative medicine

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