Quantification of Endogenous Cholesterol in Human Serum on Paper Using Direct Analysis in Real Time Mass Spectrometry

Hua Yi Hsieh, Li Hua Li, Ren Yu Hsu, Wei Fong Kao, Ying Chen Huang, Cheng Chih Hsu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Blood testing for endogenous small metabolites to determine physiological and biochemical states is routine for laboratory analysis. Here we demonstrate that by combining the commercial direct analysis in real time (DART) ion source with an ion trap mass spectrometer, native cholesterol in its free alcohol form is readily detected from a few hundred nanoliters of human serum loaded onto chromatography paper. Deuterium-labeled cholesterol was used as the internal standard to obtain the absolute quantity of the endogenous cholesterol. The amount of the cholesterol measured by this paper-loaded DART mass spectrometry (pDART-MS) is statistically comparable with that obtained by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrometry. Furthermore, sera from 21 participants at three different time points in an ultramarathon were collected to obtain their cholesterol levels. The test requires only very minimal sample preparation, and the concentrations of cholesterol in each sample were acquired within a minute.

Original languageEnglish
Pages (from-to)6146-6152
Number of pages7
JournalAnalytical Chemistry
Volume89
Issue number11
DOIs
Publication statusPublished - Jun 6 2017

ASJC Scopus subject areas

  • Analytical Chemistry

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