Abstract
Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 115-119 |
| Number of pages | 5 |
| Journal | Botanical Bulletin of Academia Sinica |
| Volume | 40 |
| Issue number | 2 |
| Publication status | Published - Apr 1999 |
| Externally published | Yes |
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Keywords
- Blockwise
- Degree of esterification (DE)
- Pectin
- Random
- Ruthenium red
ASJC Scopus subject areas
- Plant Science
Cite this
Qualitative distinction of carboxyl group distributions in pectins with ruthenium red. / Hou, Wen Chi; Chang, Wei Hsien; Jiang, Chii Ming.
In: Botanical Bulletin of Academia Sinica, Vol. 40, No. 2, 04.1999, p. 115-119.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Qualitative distinction of carboxyl group distributions in pectins with ruthenium red
AU - Hou, Wen Chi
AU - Chang, Wei Hsien
AU - Jiang, Chii Ming
PY - 1999/4
Y1 - 1999/4
N2 - Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.
AB - Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.
KW - Blockwise
KW - Degree of esterification (DE)
KW - Pectin
KW - Random
KW - Ruthenium red
UR - http://www.scopus.com/inward/record.url?scp=0032931142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032931142&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032931142
VL - 40
SP - 115
EP - 119
JO - Botanical Studies
JF - Botanical Studies
SN - 1817-406X
IS - 2
ER -