Qualitative distinction of carboxyl group distributions in pectins with ruthenium red

Wen Chi Hou, Wei Hsien Chang, Chii Ming Jiang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalBotanical Bulletin of Academia Sinica
Volume40
Issue number2
Publication statusPublished - Apr 1999
Externally publishedYes

Fingerprint

pectins
pectinesterase
sodium hydroxide
esterification
ruthenium red

Keywords

  • Blockwise
  • Degree of esterification (DE)
  • Pectin
  • Random
  • Ruthenium red

ASJC Scopus subject areas

  • Plant Science

Cite this

Qualitative distinction of carboxyl group distributions in pectins with ruthenium red. / Hou, Wen Chi; Chang, Wei Hsien; Jiang, Chii Ming.

In: Botanical Bulletin of Academia Sinica, Vol. 40, No. 2, 04.1999, p. 115-119.

Research output: Contribution to journalArticle

@article{b2a0ff3bc1f24088ade9f9a2a3964059,
title = "Qualitative distinction of carboxyl group distributions in pectins with ruthenium red",
abstract = "Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0{\%}, 31{\%}, 63{\%} and 93{\%}) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0{\%} to 80{\%}). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.",
keywords = "Blockwise, Degree of esterification (DE), Pectin, Random, Ruthenium red",
author = "Hou, {Wen Chi} and Chang, {Wei Hsien} and Jiang, {Chii Ming}",
year = "1999",
month = "4",
language = "English",
volume = "40",
pages = "115--119",
journal = "Botanical Studies",
issn = "1817-406X",
publisher = "Academia Sinica",
number = "2",

}

TY - JOUR

T1 - Qualitative distinction of carboxyl group distributions in pectins with ruthenium red

AU - Hou, Wen Chi

AU - Chang, Wei Hsien

AU - Jiang, Chii Ming

PY - 1999/4

Y1 - 1999/4

N2 - Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.

AB - Ruthenium red binding to free carboxyl groups of commercial pectins with different degrees of esterification (DE, 0%, 31%, 63% and 93%) was investigated. The optimal binding condition of ruthenium red for carboxyl groups was further studied using self-prepared pectins of different DE (0% to 80%). These were prepared by treatment with either sodium hydroxide (for random type) at 4°C or commercial pectinesterases of orange sources (for blockwise type) at 30°C. The results revealed different relationships between DE from different treatments and the corresponding ΔA534 nm value, a measure of the amount of bound ruthenium red. Negative first order (r = -0.995) and negative second order (r = -0.998) regression correlations were found, respectively, with sodium hydroxide-treated and pectinesterase-treated pectins. Ruthenium red binding to pectin might distinguish blockwise carboxyl group distributions from random ones in pectin molecules.

KW - Blockwise

KW - Degree of esterification (DE)

KW - Pectin

KW - Random

KW - Ruthenium red

UR - http://www.scopus.com/inward/record.url?scp=0032931142&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032931142&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032931142

VL - 40

SP - 115

EP - 119

JO - Botanical Studies

JF - Botanical Studies

SN - 1817-406X

IS - 2

ER -