Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus

Chee Keng Mok, Shih Cheng Chang, Guang Wu Chen, Yu Lun Lo, Shu Jen Chen, Ho Sheng Wu, Ming Tsan Liu, Feng Yee Chang, Tzou Yien Lin, Shin Ru Shih

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Prompt diagnosis of an oseltamivir-resistant marker is important for patient management, in particular to prevent the spread of resistant strains in the recent human H7N9 outbreak. We tailored a pyrosequencing assay to reveal neuraminidase R292K, a resistant marker found in one isolate from China, and demonstrated its performance in both sensitivity and specificity. In addition, a semi-nested polymerase chain reaction was applied, which enhanced the detection rate by at least 10-fold. We validated this assay by examining the marker in Taiwan's first imported human case and found R and K in quasispecies.

Original languageEnglish
Pages (from-to)465-469
Number of pages5
JournalJournal of Microbiology, Immunology and Infection
Volume48
Issue number4
DOIs
Publication statusPublished - Aug 1 2015
Externally publishedYes

Keywords

  • Avian influenza A virus
  • H7N9
  • Oseltamivir-resistant marker
  • Pyrosequencing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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  • Cite this

    Mok, C. K., Chang, S. C., Chen, G. W., Lo, Y. L., Chen, S. J., Wu, H. S., Liu, M. T., Chang, F. Y., Lin, T. Y., & Shih, S. R. (2015). Pyrosequencing reveals an oseltamivir-resistant marker in the quasispecies of avian influenza A (H7N9) virus. Journal of Microbiology, Immunology and Infection, 48(4), 465-469. https://doi.org/10.1016/j.jmii.2013.09.010