PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress

Mei Ling Kuo, Mabel Bin Er Lee, Michelle Tang, Willem Den Besten, Shuya Hu, Michael J. Sweredoski, Sonja Hess, Chih Ming Chou, Ou-Chun Chang, Mingming Su, Wei Jia, Leila Su, Yun Yen

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29 Citations (Scopus)

Abstract

Ribonucleotide reductase small subunit B (RRM2B) is a stress response protein that protects normal human fibroblasts from oxidative stress. However, the underlying mechanism that governs this function is not entirely understood. To identify factors that interact with RRM2B and mediate anti-oxidation function, large-scale purification of human Flag-tagged RRM2B complexes was performed. Pyrroline-5-carboxylate reductase 1 and 2 (PYCR1, PYCR2) were identified by mass spectrometry analysis as components of RRM2B complexes. Silencing of both PYCR1 and PYCR2 by expressing short hairpin RNAs induced defects in cell proliferation, partial fragmentation of the mitochondrial network, and hypersensitivity to oxidative stress in hTERT-immortalized human foreskin fibroblasts (HFF-hTERT). Moderate overexpression of RRM2B, comparable to stress-induced level, protected cells from oxidative stress. Silencing of both PYCR1 and PYCR2 completely abolished anti-oxidation activity of RRM2B, demonstrating a functional collaboration of these metabolic enzymes in response to oxidative stress.

Original languageEnglish
Article number18846
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Jan 6 2016

ASJC Scopus subject areas

  • General

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