Purification of human ceruloplasmin as a by-product of C1-inhibitor

Ferdinand Kouoh Elombo, Miryana Radosevich, Michel Poulle, Jacques Descamps, Sami Chtourou, Thierry Burnouf, Jean Pierre Catteau, Jean Luc Bernier, Nicole Cotelle

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Human ceruloplasmin (Cp) has been purified from cryoprecipitate-poor plasma as a by-product of the C1-inhibitor production chain. Highly purified Cp was obtained by subsequent ion-exchange chromatography on sulfate-Fractogel EMD and TMAE-Fractogel EMD. Treatments for viral safety included application of the solvent-detergent method and two nanofiltration steps using 35- and 15-nm pore size filters at the end of the process. Overall antigen yield was 95 (±5)%. Purified human ceruloplasmin was studied by electron spin resonance (ESR) to characterize its different types of copper complexes and to check its antioxidant properties. We distinguished three types of complexes: One type-2 Cu(II) with g//=2.25 and A//=180 G and two type-1 Cu(II) exhibiting different narrow hyperfine splitting (A//=72 G and A//=90 G) with close g//(2.20 and 2.21). Purified Cp has a specific activity of 24.5±0.2 mU/mg of proteins. This process provides a method for Cp purification that could be easily integrated into modern plasma fractionation.

Original languageEnglish
Pages (from-to)1406-1409
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number12
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Antioxidant
  • Ceruloplasmin
  • ESR spectroscopy
  • Reactive oxygen specie

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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