Purification of human ceruloplasmin as a by-product of C1-inhibitor

Ferdinand Kouoh Elombo, Miryana Radosevich, Michel Poulle, Jacques Descamps, Sami Chtourou, Thierry Burnouf, Jean Pierre Catteau, Jean Luc Bernier, Nicole Cotelle

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Human ceruloplasmin (Cp) has been purified from cryoprecipitate-poor plasma as a by-product of the C1-inhibitor production chain. Highly purified Cp was obtained by subsequent ion-exchange chromatography on sulfate-Fractogel EMD and TMAE-Fractogel EMD. Treatments for viral safety included application of the solvent-detergent method and two nanofiltration steps using 35- and 15-nm pore size filters at the end of the process. Overall antigen yield was 95 (±5)%. Purified human ceruloplasmin was studied by electron spin resonance (ESR) to characterize its different types of copper complexes and to check its antioxidant properties. We distinguished three types of complexes: One type-2 Cu(II) with g//=2.25 and A//=180 G and two type-1 Cu(II) exhibiting different narrow hyperfine splitting (A//=72 G and A//=90 G) with close g//(2.20 and 2.21). Purified Cp has a specific activity of 24.5±0.2 mU/mg of proteins. This process provides a method for Cp purification that could be easily integrated into modern plasma fractionation.

Original languageEnglish
Pages (from-to)1406-1409
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume23
Issue number12
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Ceruloplasmin
Ion Exchange Chromatography
Electron Spin Resonance Spectroscopy
Detergents
Sulfates
Copper
Antioxidants
Safety
Antigens
Proteins

Keywords

  • Antioxidant
  • Ceruloplasmin
  • ESR spectroscopy
  • Reactive oxygen specie

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Kouoh Elombo, F., Radosevich, M., Poulle, M., Descamps, J., Chtourou, S., Burnouf, T., ... Cotelle, N. (2000). Purification of human ceruloplasmin as a by-product of C1-inhibitor. Biological and Pharmaceutical Bulletin, 23(12), 1406-1409.

Purification of human ceruloplasmin as a by-product of C1-inhibitor. / Kouoh Elombo, Ferdinand; Radosevich, Miryana; Poulle, Michel; Descamps, Jacques; Chtourou, Sami; Burnouf, Thierry; Catteau, Jean Pierre; Bernier, Jean Luc; Cotelle, Nicole.

In: Biological and Pharmaceutical Bulletin, Vol. 23, No. 12, 2000, p. 1406-1409.

Research output: Contribution to journalArticle

Kouoh Elombo, F, Radosevich, M, Poulle, M, Descamps, J, Chtourou, S, Burnouf, T, Catteau, JP, Bernier, JL & Cotelle, N 2000, 'Purification of human ceruloplasmin as a by-product of C1-inhibitor', Biological and Pharmaceutical Bulletin, vol. 23, no. 12, pp. 1406-1409.
Kouoh Elombo F, Radosevich M, Poulle M, Descamps J, Chtourou S, Burnouf T et al. Purification of human ceruloplasmin as a by-product of C1-inhibitor. Biological and Pharmaceutical Bulletin. 2000;23(12):1406-1409.
Kouoh Elombo, Ferdinand ; Radosevich, Miryana ; Poulle, Michel ; Descamps, Jacques ; Chtourou, Sami ; Burnouf, Thierry ; Catteau, Jean Pierre ; Bernier, Jean Luc ; Cotelle, Nicole. / Purification of human ceruloplasmin as a by-product of C1-inhibitor. In: Biological and Pharmaceutical Bulletin. 2000 ; Vol. 23, No. 12. pp. 1406-1409.
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