Purification of growth hormones by reversed-phase high-performance liquid chromatography

Fu Ming Pan, Wen Chang Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume613
Issue number2
DOIs
Publication statusPublished - Apr 2 1993
Externally publishedYes

Fingerprint

High performance liquid chromatography
Growth Hormone
Purification
Electrophoresis
Sodium Dodecyl Sulfate
Fish
Agar
Proteins
Gels
Recovery

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Purification of growth hormones by reversed-phase high-performance liquid chromatography. / Pan, Fu Ming; Chang, Wen Chang.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 613, No. 2, 02.04.1993, p. 326-329.

Research output: Contribution to journalArticle

@article{72cc1bdb4f3145f48c7904cccd7ef816,
title = "Purification of growth hormones by reversed-phase high-performance liquid chromatography",
abstract = "Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43{\%} of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.",
author = "Pan, {Fu Ming} and Chang, {Wen Chang}",
year = "1993",
month = "4",
day = "2",
doi = "10.1016/0378-4347(93)80149-X",
language = "English",
volume = "613",
pages = "326--329",
journal = "Journal of Chromatography B: Biomedical Sciences and Applications",
issn = "0378-4347",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Purification of growth hormones by reversed-phase high-performance liquid chromatography

AU - Pan, Fu Ming

AU - Chang, Wen Chang

PY - 1993/4/2

Y1 - 1993/4/2

N2 - Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.

AB - Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.

UR - http://www.scopus.com/inward/record.url?scp=0027510422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027510422&partnerID=8YFLogxK

U2 - 10.1016/0378-4347(93)80149-X

DO - 10.1016/0378-4347(93)80149-X

M3 - Article

C2 - 8491820

AN - SCOPUS:0027510422

VL - 613

SP - 326

EP - 329

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

SN - 0378-4347

IS - 2

ER -