Purification of growth hormones by reversed-phase high-performance liquid chromatography

Fu Ming Pan, Wen Chang Chang

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Abstract

Reversed-phase high-performance liquid chromatography (HPLC) on a column of Radial-Pak C18 cartridge was utilized for the purification of a variety of growth hormone (GH) proteins from mammalian, avian, amphibian and fish pituitary glands. Recovery of GH from pituitary glands of up to 0.43% of total protein was obtained with a high degree of homogeneity as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The HPLC-purified GHs show reactions of identity or near identity by immuno-diffusion studies on agar gel. This method offers a convenient and rapid purification of vertebrate GH on an analytical or preparative scale.

Original languageEnglish
Pages (from-to)326-329
Number of pages4
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume613
Issue number2
DOIs
Publication statusPublished - Apr 2 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Chemistry(all)

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