Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis

Robert J. Isfort, Daniel Robinson, Hsing Jien Kung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (> 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalJournal of Virological Methods
Volume27
Issue number3
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Herpesviridae
Electrophoresis
DNA
Marek Disease
Organism Cloning
Molecular Weight
Enzymes

Keywords

  • DNA
  • Herpesvirus
  • Pulse-field electrophoresis

ASJC Scopus subject areas

  • Virology

Cite this

Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis. / Isfort, Robert J.; Robinson, Daniel; Kung, Hsing Jien.

In: Journal of Virological Methods, Vol. 27, No. 3, 01.01.1990, p. 311-317.

Research output: Contribution to journalArticle

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