Purification of carp growth hormone and cloning of the complementary DNA

Shiow Chyi Chao, Fu Ming Pan, Wen Chang Chang

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The growth hormone (GH) was isolated and purified from common carp (Cyprinus carpio) pituitary glands by salt precipitation and HPLC on reverse-phase C18 columns. The carp GH cDNA was synthesized and cloned in Escherichia coli using EcoRI linkers and pBR322 as vector. The positive clones were selected and sequenced. The full-length carp GH cDNA contains 1187 nucleotide basepairs with an open reading frame coding for the precursor form carp GH of 210 amino-acid residues. The partial amino-acid sequence from the protein completely agrees with that derived from the cDNA, with serine as the first residue in mature carp GH preceded by a 22-residue hydrophobic signal peptide. Comparison of the amino-acid sequence of carp GH with those of various species reveals positional identity at 32.4%, 38.8%, 42.0%, 37.2%, 66%, 55% and 49% with GHs of man, rat, duck, bullfrog, salmon, tuna and yellow tail, respectively.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1007
Issue number2
DOIs
Publication statusPublished - Mar 1 1989
Externally publishedYes

Fingerprint

Carps
Cloning
Growth Hormone
Purification
Organism Cloning
Complementary DNA
Amino Acids
Amino Acid Sequence
Tuna
Rana catesbeiana
Protein Sorting Signals
Ducks
Salmon
Serine
Escherichia coli
Pituitary Gland
Rats
Open Reading Frames
Nucleotides
Salts

Keywords

  • (Carp, C. carpio)
  • Amino acid sequence
  • cDNA sequence
  • Growth hormone
  • Sequence homology

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Purification of carp growth hormone and cloning of the complementary DNA. / Chao, Shiow Chyi; Pan, Fu Ming; Chang, Wen Chang.

In: BBA - Gene Structure and Expression, Vol. 1007, No. 2, 01.03.1989, p. 233-236.

Research output: Contribution to journalArticle

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