Purification of carp growth hormone and cloning of the complementary DNA

Shiow Chyi Chao, Fu Ming Pan, Wen Chang Chang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The growth hormone (GH) was isolated and purified from common carp (Cyprinus carpio) pituitary glands by salt precipitation and HPLC on reverse-phase C18 columns. The carp GH cDNA was synthesized and cloned in Escherichia coli using EcoRI linkers and pBR322 as vector. The positive clones were selected and sequenced. The full-length carp GH cDNA contains 1187 nucleotide basepairs with an open reading frame coding for the precursor form carp GH of 210 amino-acid residues. The partial amino-acid sequence from the protein completely agrees with that derived from the cDNA, with serine as the first residue in mature carp GH preceded by a 22-residue hydrophobic signal peptide. Comparison of the amino-acid sequence of carp GH with those of various species reveals positional identity at 32.4%, 38.8%, 42.0%, 37.2%, 66%, 55% and 49% with GHs of man, rat, duck, bullfrog, salmon, tuna and yellow tail, respectively.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1007
Issue number2
DOIs
Publication statusPublished - Mar 1 1989
Externally publishedYes

Keywords

  • (Carp, C. carpio)
  • Amino acid sequence
  • cDNA sequence
  • Growth hormone
  • Sequence homology

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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