Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2

Cheng Chung Lee; Tzu Ping Ko; Meng Shiou Lee; Chia Cheng Chou; Su Yuan Lai; Andrew H.J. Wang; Min Ying Wang

doi:10.1107/S090744490300859X

Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2

Cheng Chung Lee, Tzu Ping Ko, Meng Shiou Lee, Chia Cheng Chou, Su Yuan Lai, Andrew H.J. Wang, Min Ying Wang

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The rVP2H particles, formed by infectious bursal disease virus (IBDV) outer structural and primary immunogenic protein VP2, have been crystallized and the diffraction data (6 Å) showed that the rVP2H crystals belong to the cubic space group P2₁3 with a unit-cell dimension of 323.1 Å. The icosahedral symmetry of the particles is clearly seen in the self-rotation function maps, with dyads and triads in coincidence with the crystallographic axes.

Original language	English
Pages (from-to)	1234-1237
Number of pages	4
Journal	Acta Crystallographica - Section D Biological Crystallography
Volume	59
Issue number	7
DOIs	https://doi.org/10.1107/S090744490300859X
Publication status	Published - Jul 1 2003
Externally published	Yes

ASJC Scopus subject areas

Structural Biology

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Lee, C. C., Ko, T. P., Lee, M. S., Chou, C. C., Lai, S. Y., Wang, A. H. J., & Wang, M. Y. (2003). Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2. Acta Crystallographica - Section D Biological Crystallography, 59(7), 1234-1237. https://doi.org/10.1107/S090744490300859X

Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2. / Lee, Cheng Chung; Ko, Tzu Ping; Lee, Meng Shiou et al.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 7, 01.07.2003, p. 1234-1237.

Research output: Contribution to journal › Article › peer-review

Lee, CC, Ko, TP, Lee, MS, Chou, CC, Lai, SY, Wang, AHJ & Wang, MY 2003, 'Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2', Acta Crystallographica - Section D Biological Crystallography, vol. 59, no. 7, pp. 1234-1237. https://doi.org/10.1107/S090744490300859X

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abstract = "The rVP2H particles, formed by infectious bursal disease virus (IBDV) outer structural and primary immunogenic protein VP2, have been crystallized and the diffraction data (6 {\AA}) showed that the rVP2H crystals belong to the cubic space group P213 with a unit-cell dimension of 323.1 {\AA}. The icosahedral symmetry of the particles is clearly seen in the self-rotation function maps, with dyads and triads in coincidence with the crystallographic axes.",

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Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2

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