Purification and properties of fatty acid esterases from yam (Dioscorea batatas Decne) tuber

Wen C. Hou, Hsien Jung Chen, Chung Fang Chang, Yaw Huei Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using β-naphthyl myristate (C14 fatty acid ester) as a screening substrate, we purified fatty acid esterases (FAEs) from yam (Dioscorea batatas Decne) tuber. Two FAE fractions (named FAE I and FAE II) were obtained after DE-52 ion exchange chromatography and Sephadex G-75 gel filtration purification steps, and then further purified by Con A Sepharose 4B affinity chromatography. FAE I and II fractions contained the same three protein bands about 50-64 kDa corresponding to esterase activity bands on SDS-PAGE gels. Among β-naphthyl esters determined at pH 4.0, 5.0 and 6.0 the best substrate for both FAE fractions was a C10-containing one with a maximum pH at 5.0. The K(m) and V(max) for β-naphthyl caprate (C10 fatty acid ester) of FAE I and II at 37°C, pH 5.0 were 0.338 and 0.959 mM; 0.405 and 0.585 nmole β-naphthol/min μg protein, respectively. FAE activity was stable below 50°C and lost completely above 65°C.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalBotanical Bulletin of Academia Sinica
Volume40
Issue number4
Publication statusPublished - Oct 1999
Externally publishedYes

Fingerprint

Dioscorea polystachya
yams
esterases
tubers
fatty acids
fatty acid esters
gels
naphthols
ion exchange chromatography
affinity chromatography
concanavalin A
agarose
proteins
esters
screening

Keywords

  • β-naphthyl caprate
  • β-naphthyl myristate
  • Dioscorea batatas Decne
  • Fatty acid esterase (FAE)
  • Purification

ASJC Scopus subject areas

  • Plant Science

Cite this

Purification and properties of fatty acid esterases from yam (Dioscorea batatas Decne) tuber. / Hou, Wen C.; Chen, Hsien Jung; Chang, Chung Fang; Lin, Yaw Huei.

In: Botanical Bulletin of Academia Sinica, Vol. 40, No. 4, 10.1999, p. 305-310.

Research output: Contribution to journalArticle

Hou, Wen C. ; Chen, Hsien Jung ; Chang, Chung Fang ; Lin, Yaw Huei. / Purification and properties of fatty acid esterases from yam (Dioscorea batatas Decne) tuber. In: Botanical Bulletin of Academia Sinica. 1999 ; Vol. 40, No. 4. pp. 305-310.
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