Purification and properties of fatty acid esterases from yam (Dioscorea batatas Decne) tuber

Wen C. Hou, Hsien Jung Chen, Chung Fang Chang, Yaw Huei Lin

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7 Citations (Scopus)

Abstract

Using β-naphthyl myristate (C14 fatty acid ester) as a screening substrate, we purified fatty acid esterases (FAEs) from yam (Dioscorea batatas Decne) tuber. Two FAE fractions (named FAE I and FAE II) were obtained after DE-52 ion exchange chromatography and Sephadex G-75 gel filtration purification steps, and then further purified by Con A Sepharose 4B affinity chromatography. FAE I and II fractions contained the same three protein bands about 50-64 kDa corresponding to esterase activity bands on SDS-PAGE gels. Among β-naphthyl esters determined at pH 4.0, 5.0 and 6.0 the best substrate for both FAE fractions was a C10-containing one with a maximum pH at 5.0. The K(m) and V(max) for β-naphthyl caprate (C10 fatty acid ester) of FAE I and II at 37°C, pH 5.0 were 0.338 and 0.959 mM; 0.405 and 0.585 nmole β-naphthol/min μg protein, respectively. FAE activity was stable below 50°C and lost completely above 65°C.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalBotanical Bulletin of Academia Sinica
Volume40
Issue number4
Publication statusPublished - Oct 1999
Externally publishedYes

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Keywords

  • β-naphthyl caprate
  • β-naphthyl myristate
  • Dioscorea batatas Decne
  • Fatty acid esterase (FAE)
  • Purification

ASJC Scopus subject areas

  • Plant Science

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