Purification and Characterization of Reversible Sperm Motility Inhibitors from Porcine Seminal Plasma

H. Jeng, K. M. Liu, W. C. Chang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Two sperm motility inhibtors (SMI1 and SMI2) were purified from porcine seminal plasma with high performance liquid chromatography (HPLC). Their molecular weights are about 15,000 as estimated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Both of them decreased the percentage of motile spermatozoa in a dose-dependent manner. The inhibitory effect can be abolished by addition of the porcine follicular fluid. Both SMI1 and SMI2 have similar amino acid composition, suggesting that they may be structurally related. They also have inhibitory effect on chymotrypsin.

Original languageEnglish
Pages (from-to)435-440
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
Publication statusPublished - Mar 15 1993
Externally publishedYes

Fingerprint

Sperm Motility
Semen
Purification
Swine
Plasmas
Follicular Fluid
Chymotrypsin
High performance liquid chromatography
Electrophoresis
Spermatozoa
Polyacrylamide Gel Electrophoresis
Molecular Weight
Molecular weight
High Pressure Liquid Chromatography
Amino Acids
Fluids
Chemical analysis
sperm motility inhibitor 2
polyacrylamide gels

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Purification and Characterization of Reversible Sperm Motility Inhibitors from Porcine Seminal Plasma. / Jeng, H.; Liu, K. M.; Chang, W. C.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 2, 15.03.1993, p. 435-440.

Research output: Contribution to journalArticle

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