Purification and characterization of a novel cellooligosaccharide oxidase from rice pathogen Sarocladium oryzae

Meng Hwan Lee, Wen Lin Lai, Shuen Fuh Lin, Yu Liu, Yuan Hsun Hsu, Ying Chieh Tsai

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Sugar-oxidizing enzymes are widely detected in many fungal species. In this paper, a new cellooligosaccharide oxidase (COOX) was purified to homogeneity from wheat bran culture of a soil-isolated rice pathogen fungus strain, Sarocladium oryzae F137. This new sugar oxidase was composed of a single polypeptide chain with a molecular mass of 55 kDa and isoelectric point of 4.8-4.9. This enzyme contained 1 mol of FAD per mole of enzyme but no heme domain. This enzyme selectively bound to cellulose, but not to starch, chitin, or xylan. This enzyme oxidized oligosaccharides with reducing-end glucosyl residues linked by an β-1,4 glucosidic bond, such as lactose, cellobiose, and cellooligosaccharids. No significant substrate inhibition was observed at high concentrations of lactose, cellobiose, and maltose. COOX was used to produce a natural antioxidant, lactobionic acid, in a batch type reaction. The enzyme showed a preference for the two-electron acceptor 2,6-dichlorophenol-indophenol over the one-electron acceptors cytochrome c or ferricyanide. COOX did not contain a heme domain and used only two-electron acceptor. This feature could distinguish between the extracellular hemoflavoenzyme, cellobiose dehydrogenase, which is produced by a number of wood-degrading and phytopathogenic fungi.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalEnzyme and Microbial Technology
Volume39
Issue number1
DOIs
Publication statusPublished - Jun 1 2006

Keywords

  • Cellooligosaccharide oxidase
  • Characterization
  • Purification
  • Solid-state fermentation

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

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