Pulsed radiofrequency reduced complete Freund's adjuvant-induced mechanical hyperalgesia via the spinal c-Jun N-terminal kinase pathway

Kuan Hung Chen, Chien Hui Yang, Sin Ei Juang, Hui Wen Huang, Jen Kun Cheng, Shyr Ming Sheen-Chen, Jiin Tsuey Cheng, Chung Ren Lin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pulsed radiofrequency (PRF) treatment involves the pulsed application of a radiofrequency electric field to a nerve. The technology offers pain relief for patients suffering from chronic pain who do not respond well to conventional treatments. We tested whether PRF treatment attenuated complete Freund's adjuvant (CFA) induced inflammatory pain. The profile of spinal c-Jun N-terminal kinases (JNKs) phosphorylation was evaluated to elucidate the potential mechanism. Injection of CFA into the unilateral hind paw of rats induced mechanical hyperalgesia in both the ipsilateral and contralateral hind paws. We administered 500-kHz PRF treatment in 20-ms pulses, at a rate of 2 Hz (2 pulses per second) either to the sciatic nerve in the mid-thigh, or to the L4 anterior primary ramus just distal to the intervertebral foramen in both the CFA group and no-PRF group rats. Tissue samples were examined at 1, 3, 7, and 14 days following PRF treatments. Behavioral studies showed that PRF applied close to the dorsal root ganglion (DRG) significantly attenuated CFA-induced mechanical hyperalgesia compared to no-PRF group (P < .05). And western blotting revealed significant attenuation of the activation of JNK in the spinal dorsal horn compared to no-PRF group animals (P < .05). Application of PRF close to DRG provides an effective treatment for CFA-induced persistent mechanical hyperalgesia by attenuating JNK activation in the spinal dorsal horn.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalCellular and Molecular Neurobiology
Volume34
Issue number2
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

Keywords

  • Dorsal root ganglion
  • Inflammation
  • MAPK pathway
  • Mechanical allodynia
  • Spinal dorsal horn

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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