Pulsed radiofrequency reduced complete Freund's adjuvant-induced mechanical hyperalgesia via the spinal c-Jun N-terminal kinase pathway

Kuan Hung Chen, Chien Hui Yang, Sin Ei Juang, Hui Wen Huang, Jen Kun Cheng, Shyr Ming Sheen-Chen, Jiin Tsuey Cheng, Chung Ren Lin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Pulsed radiofrequency (PRF) treatment involves the pulsed application of a radiofrequency electric field to a nerve. The technology offers pain relief for patients suffering from chronic pain who do not respond well to conventional treatments. We tested whether PRF treatment attenuated complete Freund's adjuvant (CFA) induced inflammatory pain. The profile of spinal c-Jun N-terminal kinases (JNKs) phosphorylation was evaluated to elucidate the potential mechanism. Injection of CFA into the unilateral hind paw of rats induced mechanical hyperalgesia in both the ipsilateral and contralateral hind paws. We administered 500-kHz PRF treatment in 20-ms pulses, at a rate of 2 Hz (2 pulses per second) either to the sciatic nerve in the mid-thigh, or to the L4 anterior primary ramus just distal to the intervertebral foramen in both the CFA group and no-PRF group rats. Tissue samples were examined at 1, 3, 7, and 14 days following PRF treatments. Behavioral studies showed that PRF applied close to the dorsal root ganglion (DRG) significantly attenuated CFA-induced mechanical hyperalgesia compared to no-PRF group (P < .05). And western blotting revealed significant attenuation of the activation of JNK in the spinal dorsal horn compared to no-PRF group animals (P < .05). Application of PRF close to DRG provides an effective treatment for CFA-induced persistent mechanical hyperalgesia by attenuating JNK activation in the spinal dorsal horn.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalCellular and Molecular Neurobiology
Volume34
Issue number2
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

Fingerprint

Pulsed Radiofrequency Treatment
Freund's Adjuvant
JNK Mitogen-Activated Protein Kinases
Hyperalgesia
Spinal Ganglia
Pain
Sciatic Nerve
Thigh
Chronic Pain
Western Blotting
Phosphorylation
Technology
Injections
Therapeutics

Keywords

  • Dorsal root ganglion
  • Inflammation
  • MAPK pathway
  • Mechanical allodynia
  • Spinal dorsal horn

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Pulsed radiofrequency reduced complete Freund's adjuvant-induced mechanical hyperalgesia via the spinal c-Jun N-terminal kinase pathway. / Chen, Kuan Hung; Yang, Chien Hui; Juang, Sin Ei; Huang, Hui Wen; Cheng, Jen Kun; Sheen-Chen, Shyr Ming; Cheng, Jiin Tsuey; Lin, Chung Ren.

In: Cellular and Molecular Neurobiology, Vol. 34, No. 2, 01.03.2014, p. 195-203.

Research output: Contribution to journalArticle

Chen, Kuan Hung ; Yang, Chien Hui ; Juang, Sin Ei ; Huang, Hui Wen ; Cheng, Jen Kun ; Sheen-Chen, Shyr Ming ; Cheng, Jiin Tsuey ; Lin, Chung Ren. / Pulsed radiofrequency reduced complete Freund's adjuvant-induced mechanical hyperalgesia via the spinal c-Jun N-terminal kinase pathway. In: Cellular and Molecular Neurobiology. 2014 ; Vol. 34, No. 2. pp. 195-203.
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