Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells

W. A Y Tzong-Der, L. I N Hui-Yi, K. U O Daih-Huang, Shang Jie Tsai, Jia Ching Shieh, W. U. Jia-Chiun, L. E E Maw-Rong, L. I N Jen-Kun

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.

Original languageEnglish
Pages (from-to)5257-5264
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number12
DOIs
Publication statusPublished - Jun 24 2009
Externally publishedYes

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Acetyl-CoA Carboxylase
AMP-activated protein kinase
Fatty Acid Synthases
Column chromatography
1-Butanol
AMP-Activated Protein Kinases
Hep G2 Cells
G1 Phase
Cell growth
Tea
hepatoma
Chloroform
tea
Methanol
Chromatography
Rats
Hepatocellular Carcinoma
cell growth
Cell Cycle
Cells

Keywords

  • ACC
  • AMPK
  • FASN
  • Hyperlipidemia
  • Pu-erh tea

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Tzong-Der, W. A. Y., Hui-Yi, L. I. N., Daih-Huang, K. U. O., Tsai, S. J., Shieh, J. C., Jia-Chiun, W. U., ... Jen-Kun, L. I. N. (2009). Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells. Journal of Agricultural and Food Chemistry, 57(12), 5257-5264. https://doi.org/10.1021/jf900730e

Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells. / Tzong-Der, W. A Y; Hui-Yi, L. I N; Daih-Huang, K. U O; Tsai, Shang Jie; Shieh, Jia Ching; Jia-Chiun, W. U.; Maw-Rong, L. E E; Jen-Kun, L. I N.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 12, 24.06.2009, p. 5257-5264.

Research output: Contribution to journalArticle

Tzong-Der, WAY, Hui-Yi, LIN, Daih-Huang, KUO, Tsai, SJ, Shieh, JC, Jia-Chiun, WU, Maw-Rong, LEE & Jen-Kun, LIN 2009, 'Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells', Journal of Agricultural and Food Chemistry, vol. 57, no. 12, pp. 5257-5264. https://doi.org/10.1021/jf900730e
Tzong-Der, W. A Y ; Hui-Yi, L. I N ; Daih-Huang, K. U O ; Tsai, Shang Jie ; Shieh, Jia Ching ; Jia-Chiun, W. U. ; Maw-Rong, L. E E ; Jen-Kun, L. I N. / Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 12. pp. 5257-5264.
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