Pu-erh Tea Attenuates Hyperlipogenesis and Induces Hepatoma Cells Growth Arrest through Activating AMP-Activated Protein Kinase (AMPK) in Human HepG2 Cells

W. A Y Tzong-Der, L. I N Hui-Yi, K. U O Daih-Huang, Shang Jie Tsai, Jia Ching Shieh, W. U. Jia-Chiun, L. E E Maw-Rong, L. I N Jen-Kun

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50 Citations (Scopus)

Abstract

In the present study, we successively extracted the pu-erh raw tea with methanol (PR-1), chloroform (PR-2), ethyl acetate (PR-3), n-butanol (PR-4), and water (PR-5). Among these extracts, PR-3 extract contained ingredients with the most effective hypolipidemic potential and was further purified by column chromatography. Moreover, chronic administration of PR-3 provoked a significant reduction in levels of serum triglyceride and low-density lipoprotein (LDL) in rats. Our study demonstrated that fraction 5 from the PR-3 extract (PR-3-5s) showed a hypolipidemic effect in human hepatoma HepG2 cells. PR-3-5s decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) by stimulating AMP-activated protein kinase (AMPK) through the LKB1 pathway. Moreover, PR-3-5s blocked the progression of the cell cycle at the G1 phase by inducing p53 expression and in turn upregulating p21 expression.

Original languageEnglish
Pages (from-to)5257-5264
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number12
DOIs
Publication statusPublished - Jun 24 2009
Externally publishedYes

Keywords

  • ACC
  • AMPK
  • FASN
  • Hyperlipidemia
  • Pu-erh tea

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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