Proviral-activated c-erbB is leukemogenic but not sarcomagenic: Characterization of a replication-competent retrovirus containing the activated c-erbB

R. J. Pelley, C. Moscovici, S. Hughes, H. J. Kung

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Avian leukosis virus (ALV) induces erythroblastosis in chickens by integrating its DNA into the host c-erbB locus and by activating expression of truncated c-erbB transcripts. Although there is a 100% correlation of c-erbB activation with ALV-induced erythroblastosis, direct evidence that the activated c-erbB is oncogenic has not been established. We have constructed a replication-competent retrovirus containing the activated c-erbB to investigate its transforming potential. The Rous c-erbB virus (REB-c) was constructed by inserting the activated c-erbB cDNA into a Rous sarcoma virus vector in place of src. When transfected into transformed quail fibroblasts (QT6), the REB-c construct stably integrates and expresses c-erbB-specific transcripts and produces infectious virus. The REB-c retrovirus produces short-latency polyclonal erythroblastosis in chickens. However, in contrast to avian erythroblastosis virus which contains v-erbB, the REB-c construct does not transform chicken embryo fibroblasts in vitro, nor does the REB-c virus produce sarcomas when injected into the wing web of chickens. Our results provide the first direct evidence that the activated c-erbB which lacks the amino-terminal extracellular domain but which retains the entire carboxy-terminal sequences is leukemogenic but not sarcomagenic.

Original languageEnglish
Pages (from-to)1840-1844
Number of pages5
JournalJournal of Virology
Volume62
Issue number5
Publication statusPublished - Jan 1 1988
Externally publishedYes

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Retroviridae
Chickens
Avian Leukosis Virus
chickens
Avian leukosis virus
Viruses
Avian erythroblastosis virus
viruses
fibroblasts
Alpharetrovirus
Fibroblasts
Rous sarcoma virus
Quail
sarcoma
quails
Sarcoma
embryo (animal)
Embryonic Structures
Complementary DNA
loci

ASJC Scopus subject areas

  • Immunology

Cite this

Proviral-activated c-erbB is leukemogenic but not sarcomagenic : Characterization of a replication-competent retrovirus containing the activated c-erbB. / Pelley, R. J.; Moscovici, C.; Hughes, S.; Kung, H. J.

In: Journal of Virology, Vol. 62, No. 5, 01.01.1988, p. 1840-1844.

Research output: Contribution to journalArticle

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