Proviral-activated c-erbB is leukemogenic but not sarcomagenic: Characterization of a replication-competent retrovirus containing the activated c-erbB

R. J. Pelley, C. Moscovici, S. Hughes, H. J. Kung

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Avian leukosis virus (ALV) induces erythroblastosis in chickens by integrating its DNA into the host c-erbB locus and by activating expression of truncated c-erbB transcripts. Although there is a 100% correlation of c-erbB activation with ALV-induced erythroblastosis, direct evidence that the activated c-erbB is oncogenic has not been established. We have constructed a replication-competent retrovirus containing the activated c-erbB to investigate its transforming potential. The Rous c-erbB virus (REB-c) was constructed by inserting the activated c-erbB cDNA into a Rous sarcoma virus vector in place of src. When transfected into transformed quail fibroblasts (QT6), the REB-c construct stably integrates and expresses c-erbB-specific transcripts and produces infectious virus. The REB-c retrovirus produces short-latency polyclonal erythroblastosis in chickens. However, in contrast to avian erythroblastosis virus which contains v-erbB, the REB-c construct does not transform chicken embryo fibroblasts in vitro, nor does the REB-c virus produce sarcomas when injected into the wing web of chickens. Our results provide the first direct evidence that the activated c-erbB which lacks the amino-terminal extracellular domain but which retains the entire carboxy-terminal sequences is leukemogenic but not sarcomagenic.

Original languageEnglish
Pages (from-to)1840-1844
Number of pages5
JournalJournal of Virology
Volume62
Issue number5
Publication statusPublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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