Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Chun-Han Chen, Cho Hwa Liao, Ya Ling Chang, Jih Hwa Guh, Shiow Lin Pan, Che Ming Teng

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalCancer Letters
Volume315
Issue number1
DOIs
Publication statusPublished - Feb 1 2012
Externally publishedYes

Fingerprint

Excipients
Microtubules
Prostatic Neoplasms
Cell Death
CDC2 Protein Kinase
Hormones
Cell Line
Cyclin B1
Tubulin
Polymerization
Mitochondria
Down-Regulation
Phosphorylation
Apoptosis
protopine
Proteins

Keywords

  • Apoptosis
  • Cdk1
  • Microtubules
  • Mitotic arrest
  • Protopine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines. / Chen, Chun-Han; Liao, Cho Hwa; Chang, Ya Ling; Guh, Jih Hwa; Pan, Shiow Lin; Teng, Che Ming.

In: Cancer Letters, Vol. 315, No. 1, 01.02.2012, p. 1-11.

Research output: Contribution to journalArticle

@article{7588f87ad99943ebba18790f5be2aa8c,
title = "Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines",
abstract = "In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.",
keywords = "Apoptosis, Cdk1, Microtubules, Mitotic arrest, Protopine",
author = "Chun-Han Chen and Liao, {Cho Hwa} and Chang, {Ya Ling} and Guh, {Jih Hwa} and Pan, {Shiow Lin} and Teng, {Che Ming}",
year = "2012",
month = "2",
day = "1",
doi = "10.1016/j.canlet.2011.09.042",
language = "English",
volume = "315",
pages = "1--11",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

AU - Chen, Chun-Han

AU - Liao, Cho Hwa

AU - Chang, Ya Ling

AU - Guh, Jih Hwa

AU - Pan, Shiow Lin

AU - Teng, Che Ming

PY - 2012/2/1

Y1 - 2012/2/1

N2 - In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

AB - In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

KW - Apoptosis

KW - Cdk1

KW - Microtubules

KW - Mitotic arrest

KW - Protopine

UR - http://www.scopus.com/inward/record.url?scp=81955167334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81955167334&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2011.09.042

DO - 10.1016/j.canlet.2011.09.042

M3 - Article

C2 - 22033245

AN - SCOPUS:81955167334

VL - 315

SP - 1

EP - 11

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -