Protopine, a novel microtubule-stabilizing agent, causes mitotic arrest and apoptotic cell death in human hormone-refractory prostate cancer cell lines

Chun-Han Chen, Cho Hwa Liao, Ya Ling Chang, Jih Hwa Guh, Shiow Lin Pan, Che Ming Teng

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

In this study, we investigated the anticancer effect of protopine on human hormone-refractory prostate cancer (HRPC) cells. Protopine exhibited an anti-proliferative effect by induction of tubulin polymerization and mitotic arrest, which ultimately led to apoptotic cell death. The data suggest that protopine increased the activity of cyclin-dependent kinase 1 (Cdk1)/cyclin B1 complex and that contributed to cell apoptosis by modulating mitochondria-mediated signaling pathways, such as Bcl-2 phosphorylation and Mcl-1 down-regulation. In conclusion, the data suggest that protopine is a novel microtubule stabilizer with anticancer activity in HRPC cells through apoptotic pathway by modulating Cdk1 activity and Bcl-2 family of proteins.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalCancer Letters
Volume315
Issue number1
DOIs
Publication statusPublished - Feb 1 2012
Externally publishedYes

Keywords

  • Apoptosis
  • Cdk1
  • Microtubules
  • Mitotic arrest
  • Protopine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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