Proton pump-rich cell secretes acid in skin of zebrafish larvae

Li Yih Lin, Jiun Lin Horng, Joseph G. Kunkel, Pung Pung Hwang

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The mammalian kidney excretes its metabolic acid load through the proton-transporting cells, intercalated cells, in the distal nephron and collecting duct. Fish excrete acid through external organs, gill, or skin; however, the cellular function is still controversial. In this study, molecular and electrophysiological approaches were used to identify a novel cell type secreting acid in skin of zebrafish (Danio rerio) larvae. Among keratinocytes covering the larval surface, novel proton-secreting ionocytes, proton pump (H+-ATPase)-rich cells, were identified to generate strong outward H+ flux. The present work demonstrates for the first time, with a noninvasive technique, H+-secreting cells in an intact animal model, the zebrafish, showing it to be a suitable model in which to study the functions of vertebrate transporting epithelia in vivo.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume290
Issue number2
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Proton Pumps
Zebrafish
Larva
Skin
Acids
Protons
Proton-Translocating ATPases
Ducts
Fish
Animals
Fluxes
Nephrons
Keratinocytes
Vertebrates
Fishes
Epithelium
Animal Models
Kidney

Keywords

  • Epithelial transport
  • Hydrogen-adenosinetriphosphatase
  • Ion-selective electrode
  • Ionocytes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Proton pump-rich cell secretes acid in skin of zebrafish larvae. / Lin, Li Yih; Horng, Jiun Lin; Kunkel, Joseph G.; Hwang, Pung Pung.

In: American Journal of Physiology - Cell Physiology, Vol. 290, No. 2, 02.2006.

Research output: Contribution to journalArticle

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