Proteomics analysis of altered proteins in kidney of mice with aristolochic acid nephropathy using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry method

Chia En Lin, Wen Shin Chang, Jen Ai Lee, Ting Ya Chang, Yu Shen Huang, Yoshiro Hirasaki, Hung Shing Chen, Kazuhiro Imai, Shih Ming Chen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Aristolochic acid (AA) causes interstitial renal fibrosis, called aristolochic acid nephropathy (AAN). There is no specific indicator for diagnosing AAN, so this study aimed to investigate the biomarkers for AAN using a proteomics method. The C3H/He female mice were given ad libitum AA-distilled water (0.5mg/kg/day) and distilled water for 56days in the AA and normal groups, respectively. The AA-induced proteins in the kidney were investigated using a proteomics study, including fluorogenic derivatization with 7-chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide, followed by high-performance liquid chromatography analysis and liquid chromatography tandem mass spectrometry with a MASCOT database searching system. There were two altered proteins, thrombospondin type 1 (TSP1) and G protein-coupled receptor 87 (GPR87), in the kidney of AA-group mice on day 56. GPR87, a tumorigenesis-related protein, is reported for the first time in the current study. The renal interstitial fibrosis was certainly induced in the AA-group mice under histological examination. Based on the results of histological examination and the proteomics study, this model might be applied to AAN studies in the future. TSP1 might be a novel biomarker for AAN, and the further role of GPR87 leading to AA-induced tumorigenesis should be researched in future studies.

Original languageEnglish
Pages (from-to)e4127
JournalBiomedical Chromatography
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Keywords

  • Aristolochic acid nephropathy (AAN)
  • DAABD-cl
  • FD-LC-MS/MS
  • G protein-coupled receptor 87 (GPR87)
  • Proteomics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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