Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’

Lixiao Yao, Qibin Yu, Ming Huang, Weilun Hung, Jude Grosser, Shanchun Chen, Yu Wang, Frederick G. Gmitter

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Orange fruit from trees infected by ‘Candidatus Liberibacter asiaticus’ (CaLas) often do not look fully mature and exhibit off-flavours described as bitter, harsh, and metallic rather than juicy and fruity. Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods, the mechanisms leading to the off-flavour that occurs in Huanglongbing (HLB)-symptomatic fruit are not well understood. In this study, fruits were collected from symptomatic and healthy Valencia sweet orange (Citrus sinensis) trees grafted on Swingle (C. paradisi X Poncirus trifoliata) rootstock. Isobaric tags for relative and absolute quantification (iTRAQ) and gas chromatography-mass spectrometry (GC-MS) were used to measure the proteins, sugars, organic acids, amino acids, and volatile terpenoids. The results showed that most of the differentially expressed proteins involved in glycolysis, the tricarboxylic acid (TCA) cycle and amino-acid biosynthesis were degraded, and terpenoid metabolism was significantly downregulated in the symptomatic fruit. Valencene, limonene, 3-carene, linalool, myrcene, and α-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy trees. Similar phenomena were observed for sucrose and glucose. Our study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis, the TCA cycle, and the terpenoid biosynthesis pathway.

Original languageEnglish
Article number31
JournalHorticulture Research
Volume6
Issue number1
DOIs
Publication statusPublished - Dec 1 2019
Externally publishedYes

Fingerprint

Candidatus Liberibacter asiaticus
Metabolomics
Citrus
Flavors
off flavors
metabolomics
Fruits
Proteomics
proteomics
Fruit
Terpenes
terpenoids
fruits
Citric Acid Cycle
tricarboxylic acid cycle
Biosynthesis
glycolysis
Glycolysis
Poncirus
Down-Regulation

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’. / Yao, Lixiao; Yu, Qibin; Huang, Ming; Hung, Weilun; Grosser, Jude; Chen, Shanchun; Wang, Yu; Gmitter, Frederick G.

In: Horticulture Research, Vol. 6, No. 1, 31, 01.12.2019.

Research output: Contribution to journalArticle

Yao, Lixiao ; Yu, Qibin ; Huang, Ming ; Hung, Weilun ; Grosser, Jude ; Chen, Shanchun ; Wang, Yu ; Gmitter, Frederick G. / Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’. In: Horticulture Research. 2019 ; Vol. 6, No. 1.
@article{7dfeb5b4da9f4f5aa90497e2f01405de,
title = "Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’",
abstract = "Orange fruit from trees infected by ‘Candidatus Liberibacter asiaticus’ (CaLas) often do not look fully mature and exhibit off-flavours described as bitter, harsh, and metallic rather than juicy and fruity. Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods, the mechanisms leading to the off-flavour that occurs in Huanglongbing (HLB)-symptomatic fruit are not well understood. In this study, fruits were collected from symptomatic and healthy Valencia sweet orange (Citrus sinensis) trees grafted on Swingle (C. paradisi X Poncirus trifoliata) rootstock. Isobaric tags for relative and absolute quantification (iTRAQ) and gas chromatography-mass spectrometry (GC-MS) were used to measure the proteins, sugars, organic acids, amino acids, and volatile terpenoids. The results showed that most of the differentially expressed proteins involved in glycolysis, the tricarboxylic acid (TCA) cycle and amino-acid biosynthesis were degraded, and terpenoid metabolism was significantly downregulated in the symptomatic fruit. Valencene, limonene, 3-carene, linalool, myrcene, and α-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy trees. Similar phenomena were observed for sucrose and glucose. Our study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis, the TCA cycle, and the terpenoid biosynthesis pathway.",
author = "Lixiao Yao and Qibin Yu and Ming Huang and Weilun Hung and Jude Grosser and Shanchun Chen and Yu Wang and Gmitter, {Frederick G.}",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41438-018-0109-z",
language = "English",
volume = "6",
journal = "Horticulture Research",
issn = "2052-7276",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Proteomic and metabolomic analyses provide insight into the off-flavour of fruits from citrus trees infected with ‘Candidatus Liberibacter asiaticus’

AU - Yao, Lixiao

AU - Yu, Qibin

AU - Huang, Ming

AU - Hung, Weilun

AU - Grosser, Jude

AU - Chen, Shanchun

AU - Wang, Yu

AU - Gmitter, Frederick G.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Orange fruit from trees infected by ‘Candidatus Liberibacter asiaticus’ (CaLas) often do not look fully mature and exhibit off-flavours described as bitter, harsh, and metallic rather than juicy and fruity. Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods, the mechanisms leading to the off-flavour that occurs in Huanglongbing (HLB)-symptomatic fruit are not well understood. In this study, fruits were collected from symptomatic and healthy Valencia sweet orange (Citrus sinensis) trees grafted on Swingle (C. paradisi X Poncirus trifoliata) rootstock. Isobaric tags for relative and absolute quantification (iTRAQ) and gas chromatography-mass spectrometry (GC-MS) were used to measure the proteins, sugars, organic acids, amino acids, and volatile terpenoids. The results showed that most of the differentially expressed proteins involved in glycolysis, the tricarboxylic acid (TCA) cycle and amino-acid biosynthesis were degraded, and terpenoid metabolism was significantly downregulated in the symptomatic fruit. Valencene, limonene, 3-carene, linalool, myrcene, and α-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy trees. Similar phenomena were observed for sucrose and glucose. Our study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis, the TCA cycle, and the terpenoid biosynthesis pathway.

AB - Orange fruit from trees infected by ‘Candidatus Liberibacter asiaticus’ (CaLas) often do not look fully mature and exhibit off-flavours described as bitter, harsh, and metallic rather than juicy and fruity. Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods, the mechanisms leading to the off-flavour that occurs in Huanglongbing (HLB)-symptomatic fruit are not well understood. In this study, fruits were collected from symptomatic and healthy Valencia sweet orange (Citrus sinensis) trees grafted on Swingle (C. paradisi X Poncirus trifoliata) rootstock. Isobaric tags for relative and absolute quantification (iTRAQ) and gas chromatography-mass spectrometry (GC-MS) were used to measure the proteins, sugars, organic acids, amino acids, and volatile terpenoids. The results showed that most of the differentially expressed proteins involved in glycolysis, the tricarboxylic acid (TCA) cycle and amino-acid biosynthesis were degraded, and terpenoid metabolism was significantly downregulated in the symptomatic fruit. Valencene, limonene, 3-carene, linalool, myrcene, and α-terpineol levels were significantly lower in fruit from CaLas-infected trees than from healthy trees. Similar phenomena were observed for sucrose and glucose. Our study indicated that off-flavour of symptomatic fruit was associated with a reduction in the levels of terpenoid products and the downregulation of proteins in glycolysis, the TCA cycle, and the terpenoid biosynthesis pathway.

UR - http://www.scopus.com/inward/record.url?scp=85061693545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061693545&partnerID=8YFLogxK

U2 - 10.1038/s41438-018-0109-z

DO - 10.1038/s41438-018-0109-z

M3 - Article

AN - SCOPUS:85061693545

VL - 6

JO - Horticulture Research

JF - Horticulture Research

SN - 2052-7276

IS - 1

M1 - 31

ER -