Proteomic analysis of post-hemorrhagic shock mesenteric lymph

Jen Feng Fang, Lih Yuann Shih, Kuo Ching Yuan, Kuan Ying Fang, Tsann Long Hwang, Sen Yung Hsieh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recent studies have documented the association of mesenteric lymphatic route with adult respiratory distress syndrome and multiple organ failure after hemorrhagic shock. However, the mediators and mechanisms of the toxic effects of mesenteric lymph remain unclear. This study aimed to identify mediators or biomarkers in the mesenteric lymph through comparative proteomic analysis. Fourteen mature male Sprague-Dawley rats were randomly divided and subjected to trauma (laparotomy) plus hemorrhagic shock or trauma plus sham shock. Mesenteric lymph samples were collected before shock and at 3 h after resuscitation from hemorrhagic shock (or sham shock). To investigate changes in proteome profiles between preshock and 3-h postshock (or 3-h post-sham shock) mesenteric lymph samples, two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry were performed. We found a more than 2-fold change in abundance of 31 protein spots in the lymph samples. Mass spectrometry analyses identified 12 distinct proteins. Four proteins were consistently upregulated in the 3-h postshock lymph samples, including serum albumin precursor, two isoforms of cytoplasmic actin, complement C3 precursor, and major urinary protein precursor. Two proteins, including haptoglobin and one unidentified protein, were consistently downregulated. The deregulation of these proteins was confirmed by Western blots. Most of these altered proteins are functionally implicated in tissue inflammation. The findings of this study provide a starting point for investigating the functions of these proteins in hemorrhagic shock-induced lung injury and hold great promise for the development of potential therapeutic interventions.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalShock
Volume34
Issue number3
DOIs
Publication statusPublished - Sep 1 2010
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Lymph
Proteomics
Shock
Proteins
Adult Respiratory Distress Syndrome
Mass Spectrometry
Complement C3
Haptoglobins
Protein Precursors
Multiple Organ Failure
Poisons
Electrophoresis, Gel, Two-Dimensional
Wounds and Injuries
Lung Injury
Proteome
Resuscitation
Serum Albumin
Laparotomy
Sprague Dawley Rats

Keywords

  • Adult respiratory distress syndrome
  • comparative proteomics
  • hemorrhagic shock
  • mesenteric lymph
  • twodimensional gel electrophoresis

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Fang, J. F., Shih, L. Y., Yuan, K. C., Fang, K. Y., Hwang, T. L., & Hsieh, S. Y. (2010). Proteomic analysis of post-hemorrhagic shock mesenteric lymph. Shock, 34(3), 291-298. https://doi.org/10.1097/SHK.0b013e3181ceef5e

Proteomic analysis of post-hemorrhagic shock mesenteric lymph. / Fang, Jen Feng; Shih, Lih Yuann; Yuan, Kuo Ching; Fang, Kuan Ying; Hwang, Tsann Long; Hsieh, Sen Yung.

In: Shock, Vol. 34, No. 3, 01.09.2010, p. 291-298.

Research output: Contribution to journalArticle

Fang, JF, Shih, LY, Yuan, KC, Fang, KY, Hwang, TL & Hsieh, SY 2010, 'Proteomic analysis of post-hemorrhagic shock mesenteric lymph', Shock, vol. 34, no. 3, pp. 291-298. https://doi.org/10.1097/SHK.0b013e3181ceef5e
Fang, Jen Feng ; Shih, Lih Yuann ; Yuan, Kuo Ching ; Fang, Kuan Ying ; Hwang, Tsann Long ; Hsieh, Sen Yung. / Proteomic analysis of post-hemorrhagic shock mesenteric lymph. In: Shock. 2010 ; Vol. 34, No. 3. pp. 291-298.
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