Proteomic analysis of hypothalamic injury in heatstroke rats

Chien Ming Chao, Bor Chih Cheng, Chia Ying Chen, Mao Tsun Lin, Ching Ping Chang, Shun Tai Yang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ischemic and oxidative damage to the hypothalamus may be associated with decreased heat tolerance as well as heatstroke formation. The present study explores the hypothalamic proteome mechanisms associated with heatstroke-mediated hypothalamic ischemia, and oxidative damage. Heatstroke rats had hypotension, hypothalamic ischemia, and lethality. In addition, they had hyperthermia and hypothalamic blood-brain-barrier disruption, oxidative stress, activated inflammation, and neuronal apoptosis and degeneration. 2DE combined LC-MS/MS revealed that heatstroke-induced ischemic injury and apoptosis were associated with upregulation of L-lactate dehydrogenase but downregulation of both dihydropyriminase-related protein and 14-3-3 Zeta isoform protein. Heat-induced blood-brain-barrier disruption might be related to upregulation of glial fibrillary acidic protein. Oxidative stress caused by heatstroke might be related to upregulation of cytosolic dehydrogenase-1. Also, heat-induced overproduction of proinflammatory cytokines might be associated with downregulation of stathmin 1. Heat-induced hypothalamic ischemia, apoptosis, injury (or upregulation of L-lactate dehydrogenase), blood-brain-barrier disruption (or upregulation of glial fibrillary acidic protein), oxidative stress (or upregulation of cytosolic dehydrogenase-1), and activated inflammation (or downregulation of stathmin 1) were all significantly reversed by whole body cooling. Our data indicate that cooling therapy improves outcomes of heatstroke by modulating hypothalamic proteome mechanisms.

Original languageEnglish
Pages (from-to)1921-1934
Number of pages14
JournalProteomics
Volume15
Issue number11
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Heat Stroke
Proteomics
Oxidative stress
Rats
Up-Regulation
Stathmin
Glial Fibrillary Acidic Protein
Wounds and Injuries
Proteome
Apoptosis
Blood-Brain Barrier
L-Lactate Dehydrogenase
14-3-3 Proteins
Oxidoreductases
Oxidative Stress
Down-Regulation
Ischemia
Hot Temperature
Cooling
Inflammation

Keywords

  • Animal proteomics
  • Body cooling
  • Heat stroke
  • Hypothalamus

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Chao, C. M., Cheng, B. C., Chen, C. Y., Lin, M. T., Chang, C. P., & Yang, S. T. (2015). Proteomic analysis of hypothalamic injury in heatstroke rats. Proteomics, 15(11), 1921-1934. https://doi.org/10.1002/pmic.201400492

Proteomic analysis of hypothalamic injury in heatstroke rats. / Chao, Chien Ming; Cheng, Bor Chih; Chen, Chia Ying; Lin, Mao Tsun; Chang, Ching Ping; Yang, Shun Tai.

In: Proteomics, Vol. 15, No. 11, 01.06.2015, p. 1921-1934.

Research output: Contribution to journalArticle

Chao, CM, Cheng, BC, Chen, CY, Lin, MT, Chang, CP & Yang, ST 2015, 'Proteomic analysis of hypothalamic injury in heatstroke rats', Proteomics, vol. 15, no. 11, pp. 1921-1934. https://doi.org/10.1002/pmic.201400492
Chao CM, Cheng BC, Chen CY, Lin MT, Chang CP, Yang ST. Proteomic analysis of hypothalamic injury in heatstroke rats. Proteomics. 2015 Jun 1;15(11):1921-1934. https://doi.org/10.1002/pmic.201400492
Chao, Chien Ming ; Cheng, Bor Chih ; Chen, Chia Ying ; Lin, Mao Tsun ; Chang, Ching Ping ; Yang, Shun Tai. / Proteomic analysis of hypothalamic injury in heatstroke rats. In: Proteomics. 2015 ; Vol. 15, No. 11. pp. 1921-1934.
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