Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon

Vorrapon Chaikeeratisak, Kunlaya Somboonwiwat, Hao Ching Wang, Chu Fang Lo, Anchalee Tassanakajon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The protein expression profiles of the lymphoid organ, taken from mock and systemic Vibrio harveyiinfected Penaeus monodon at 6 and 48 h post infection, were revealed. The considerable changes in the expression level of several proteins were observed between the mock and V. harveyi-infected shrimps. From 30 analyzed protein spots with 27 differentially expressed, 21 were known proteins with the most common of these being cytoskeleton proteins (33%) which were all down-regulated upon systemic bacterial infection. Other six proteins including four proteins that are involved in the shrimp immunity (alpha-2- macroglobulin, transglutaminase, heat shock protein 1 and hemocyanin subunit Y), and two proteins that are involved in metabolism (triosephosphate isomerase) and cell signaling (14-3-3 like protein), displayed significantly decreased expression levels. There was, however, an increase in the expression level of the ATP synthase beta subunit, a protein involved in energy balance. Transcription levels of ATP synthase beta subunit and 14-3-3 like protein were up- and down-regulated, respectively, in accord with the observed protein expression levels, but the alpha-2-macroglobulin transcript levels were significantly increased in contrast to the decreased protein expression levels. Interestingly, partial gene silencing of ATP synthase beta subunit revealed a high cumulative mortality of the knockdown shrimps (73.3%) and a dramatic reduction of the total hemocyte numbers in the survival shrimps. These altered proteins are likely to play essential roles in shrimp defense against the pathogenic bacterium V. harveyi.

Original languageEnglish
Pages (from-to)6367-6377
Number of pages11
JournalMolecular Biology Reports
Volume39
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

Penaeidae
Vibrio
Proteomics
Proteins
14-3-3 Proteins
alpha-Macroglobulins
Adenosine Triphosphate
Triose-Phosphate Isomerase
Hemocytes
Hemocyanin
Transglutaminases
Protein Subunits
Gene Silencing
Heat-Shock Proteins
Cytoskeleton
Bacterial Infections
Immunity

Keywords

  • ATP synthase beta subunit
  • Hemocyte production
  • Lymphoid organ
  • Penaeus monodon
  • Proteomics
  • Vibrio harveyi

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon. / Chaikeeratisak, Vorrapon; Somboonwiwat, Kunlaya; Wang, Hao Ching; Lo, Chu Fang; Tassanakajon, Anchalee.

In: Molecular Biology Reports, Vol. 39, No. 5, 05.2012, p. 6367-6377.

Research output: Contribution to journalArticle

Chaikeeratisak, Vorrapon ; Somboonwiwat, Kunlaya ; Wang, Hao Ching ; Lo, Chu Fang ; Tassanakajon, Anchalee. / Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon. In: Molecular Biology Reports. 2012 ; Vol. 39, No. 5. pp. 6367-6377.
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