Proteome analysis of altered proteins in streptozotocin-induced diabetic rat kidney using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry method

Pei Yun Tsai, Shih Ming Chen, Hsiang Yin Chen, Yi Chieh Li, Kazuhiro Imai, Kuang Yang Hsu, Jen Ai Lee

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Abstract

To find new molecular markers for early diagnosis of diabetic nephropathy, we applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry to identify the differentially expressed proteins in the kidney of control and streptozotocin-induced diabetic rats. The Sprague-Dawley rats were injected with the sodium citrate buffer or streptozotocin and then killed after 1, 4, 12 and 24 weeks. The results showed that seven proteins were significantly changed after 1 week of injection. Only one protein had significantly changed after 4 weeks of injection. However, after 12 weeks of injection, the number of altered proteins rose to 10. After 24 weeks of injection, 18 proteins had altered significantly. Five common proteins were significantly altered at week 12 and 24 after injection, respectively. Importantly, these proteins appeared prior to microalbuminuria and may serve as new biomarkers that are able to improve early detection of and new drug development for diabetic-related nephropathy.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalBiomedical Chromatography
Volume27
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Liquid chromatography
Proteome
Streptozocin
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Rats
Kidney
Injections
Proteins
Diabetic Nephropathies
Biomarkers
Sprague Dawley Rats
Early Diagnosis
Buffers
Pharmaceutical Preparations

Keywords

  • DAABD-Cl
  • Diabetic nephropathy
  • FD-LC-MS/MS method
  • Fluorogenic derivatization

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

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title = "Proteome analysis of altered proteins in streptozotocin-induced diabetic rat kidney using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry method",
abstract = "To find new molecular markers for early diagnosis of diabetic nephropathy, we applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry to identify the differentially expressed proteins in the kidney of control and streptozotocin-induced diabetic rats. The Sprague-Dawley rats were injected with the sodium citrate buffer or streptozotocin and then killed after 1, 4, 12 and 24 weeks. The results showed that seven proteins were significantly changed after 1 week of injection. Only one protein had significantly changed after 4 weeks of injection. However, after 12 weeks of injection, the number of altered proteins rose to 10. After 24 weeks of injection, 18 proteins had altered significantly. Five common proteins were significantly altered at week 12 and 24 after injection, respectively. Importantly, these proteins appeared prior to microalbuminuria and may serve as new biomarkers that are able to improve early detection of and new drug development for diabetic-related nephropathy.",
keywords = "DAABD-Cl, Diabetic nephropathy, FD-LC-MS/MS method, Fluorogenic derivatization",
author = "Tsai, {Pei Yun} and Chen, {Shih Ming} and Chen, {Hsiang Yin} and Li, {Yi Chieh} and Kazuhiro Imai and Hsu, {Kuang Yang} and Lee, {Jen Ai}",
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AU - Tsai, Pei Yun

AU - Chen, Shih Ming

AU - Chen, Hsiang Yin

AU - Li, Yi Chieh

AU - Imai, Kazuhiro

AU - Hsu, Kuang Yang

AU - Lee, Jen Ai

PY - 2013/3

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N2 - To find new molecular markers for early diagnosis of diabetic nephropathy, we applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry to identify the differentially expressed proteins in the kidney of control and streptozotocin-induced diabetic rats. The Sprague-Dawley rats were injected with the sodium citrate buffer or streptozotocin and then killed after 1, 4, 12 and 24 weeks. The results showed that seven proteins were significantly changed after 1 week of injection. Only one protein had significantly changed after 4 weeks of injection. However, after 12 weeks of injection, the number of altered proteins rose to 10. After 24 weeks of injection, 18 proteins had altered significantly. Five common proteins were significantly altered at week 12 and 24 after injection, respectively. Importantly, these proteins appeared prior to microalbuminuria and may serve as new biomarkers that are able to improve early detection of and new drug development for diabetic-related nephropathy.

AB - To find new molecular markers for early diagnosis of diabetic nephropathy, we applied fluorogenic derivatization-liquid chromatography-tandem mass spectrometry to identify the differentially expressed proteins in the kidney of control and streptozotocin-induced diabetic rats. The Sprague-Dawley rats were injected with the sodium citrate buffer or streptozotocin and then killed after 1, 4, 12 and 24 weeks. The results showed that seven proteins were significantly changed after 1 week of injection. Only one protein had significantly changed after 4 weeks of injection. However, after 12 weeks of injection, the number of altered proteins rose to 10. After 24 weeks of injection, 18 proteins had altered significantly. Five common proteins were significantly altered at week 12 and 24 after injection, respectively. Importantly, these proteins appeared prior to microalbuminuria and may serve as new biomarkers that are able to improve early detection of and new drug development for diabetic-related nephropathy.

KW - DAABD-Cl

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KW - FD-LC-MS/MS method

KW - Fluorogenic derivatization

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