Protein-tyrosine phosphatase D1, a potential regulator and effector for Tec family kinases

Hsiang Yiang Jui, Rong Jeng Tseng, Xin Wen, Hsin I. Fang, Li Ming Huang, Kai Yun Chen, Hsing Jien Kung, David K. Ann, Hsiu Ming Shih

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Etk, also named Bmx, is a member of the Tec tyrosine kinase family, which is characterized by a multimodular structure including a pleckstrin homology (PH) domain, an SH3 domain, an SH2 domain, and a catalytic domain. The signaling mechanisms regulating Etk kinase activity remain largely unknown. To identify factor(s) regulating Etk activity, we used the PH domain and a linker region of Etk as a bait for a yeast two-hybrid screen. Three independent clones encoding protein-tyrosine phosphatase D1 (PTPDI) fragments were isolated. The binding of PTPD1 to Etk is specific since PTPD1 cannot associate with either the Akt PH domain or lamin. In vitro and in vivo binding studies demonstrated that PTPD1 can interact with Etk and that residues 726-848 of PTPD1 are essential for this interaction. Deletion analysis of Etk indicated that the PH domain is essential for PTPDI interaction. Furthermore, the Etk-PTPD1 interaction stimulated the kinase activity of Etk, resulting in an increased phosphotyrosine content in both factors. The Etk-PTPD1 interaction also increased Stat3 activation. The effect of PTPD1 on Etk activation is specific since PTPD1 cannot potentiate Jak2 activity upon Stat3 activation. In addition, Tec (but not Btk) kinase can also be activated by PTPDI. Taken together, these findings indicate that PTPD1 can selectively associate with and stimulate Tec family kinases and modulate Stat3 activation.

Original languageEnglish
Pages (from-to)41124-41132
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number52
DOIs
Publication statusPublished - Dec 29 2000
Externally publishedYes

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Protein Tyrosine Phosphatases
Phosphotransferases
Chemical activation
src Homology Domains
Lamins
Phosphotyrosine
Yeast
Catalytic Domain
Thermodynamic properties
Clone Cells
Yeasts
platelet protein P47
Tec protein-tyrosine kinase
Pleckstrin Homology Domains

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jui, H. Y., Tseng, R. J., Wen, X., Fang, H. I., Huang, L. M., Chen, K. Y., ... Shih, H. M. (2000). Protein-tyrosine phosphatase D1, a potential regulator and effector for Tec family kinases. Journal of Biological Chemistry, 275(52), 41124-41132. https://doi.org/10.1074/jbc.M007772200

Protein-tyrosine phosphatase D1, a potential regulator and effector for Tec family kinases. / Jui, Hsiang Yiang; Tseng, Rong Jeng; Wen, Xin; Fang, Hsin I.; Huang, Li Ming; Chen, Kai Yun; Kung, Hsing Jien; Ann, David K.; Shih, Hsiu Ming.

In: Journal of Biological Chemistry, Vol. 275, No. 52, 29.12.2000, p. 41124-41132.

Research output: Contribution to journalArticle

Jui, HY, Tseng, RJ, Wen, X, Fang, HI, Huang, LM, Chen, KY, Kung, HJ, Ann, DK & Shih, HM 2000, 'Protein-tyrosine phosphatase D1, a potential regulator and effector for Tec family kinases', Journal of Biological Chemistry, vol. 275, no. 52, pp. 41124-41132. https://doi.org/10.1074/jbc.M007772200
Jui, Hsiang Yiang ; Tseng, Rong Jeng ; Wen, Xin ; Fang, Hsin I. ; Huang, Li Ming ; Chen, Kai Yun ; Kung, Hsing Jien ; Ann, David K. ; Shih, Hsiu Ming. / Protein-tyrosine phosphatase D1, a potential regulator and effector for Tec family kinases. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 52. pp. 41124-41132.
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