Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection

Hao Ching Wang, Han Ching Wang, Jiann Horng Leu, Guang Hsiung Kou, Andrew H J Wang, Chu Fang Lo

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

To better understand the pathogenesis of white spot syndrome virus (WSSV) and to determine which cell pathways might be affected after WSSV infection, two-dimensional gel electrophoresis (2-DE) was used to produce protein expression profiles from samples taken at 48 h post-infection (hpi) from the stomachs of Litopenaeus vannamei (also called Penaeus vannamei) that were either specific pathogen free or else infected with WSSV. Seventy-five protein spots that consistently showed either a marked change (>50%) in accumulated levels or else were highly expressed throughout the course of WSSV infection were selected for further study. After in-gel trypsin digestion followed by LC-nanoESI-MS/MS, bioinformatics databases were searched for matches. A total of 53 proteins were identified, with functions that included energy production, calcium homeostasis, nucleic acid synthesis, signaling/communication, oxygen carrier/transportation, and SUMO-related modification. 2-DE results were shown to be consistent with relative EST database data from a previously developed EST database of two Penaeus monodon cDNA libraries. For seven selected genes, 2-DE and EST data were also compared with transcriptional time-course RT-PCR data. This study is the first global analysis of differentially expressed proteins in WSSV-infected shrimp, and in addition to increasing our understanding of the molecular pathogenesis of this virus-associated shrimp disease, the results presented here should be useful both for identifying potential biomarkers and for developing antiviral measures.

Original languageEnglish
Pages (from-to)672-686
Number of pages15
JournalDevelopmental and Comparative Immunology
Volume31
Issue number7
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

White spot syndrome virus 1
Virus Diseases
Expressed Sequence Tags
Penaeidae
Databases
Proteins
Specific Pathogen-Free Organisms
Electrophoresis, Gel, Two-Dimensional
Computational Biology
Gene Library
Trypsin
Nucleic Acids
Antiviral Agents
Digestion
Stomach
Homeostasis
Biomarkers
Gels
Communication
Oxygen

Keywords

  • Host response
  • Protein expression profiling
  • Proteomic analysis
  • White spot syndrome virus
  • WSSV infection

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection. / Wang, Hao Ching; Wang, Han Ching; Leu, Jiann Horng; Kou, Guang Hsiung; Wang, Andrew H J; Lo, Chu Fang.

In: Developmental and Comparative Immunology, Vol. 31, No. 7, 2007, p. 672-686.

Research output: Contribution to journalArticle

Wang, Hao Ching ; Wang, Han Ching ; Leu, Jiann Horng ; Kou, Guang Hsiung ; Wang, Andrew H J ; Lo, Chu Fang. / Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection. In: Developmental and Comparative Immunology. 2007 ; Vol. 31, No. 7. pp. 672-686.
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